Obestatin stimulates differentiation and regulates lipolysis and leptin secretion in rat preadipocytes

Wojciechowicz,Tatiana; Skrzypski,Marek; Kołodziejski,Paweł  A.; Szczepankiewicz,Dawid; Pruszyńska‑Oszmałek,Ewa; Kaczmarek,Przemysław; Strowski,Mathias  Z.; Nowak,Krzysztof  W.

doi:10.3892/mmr.2015.4470

Obestatin stimulates differentiation and regulates lipolysis and leptin secretion in rat preadipocytes

Authors:
- Tatiana Wojciechowicz
- Marek Skrzypski
- Paweł A. Kołodziejski
- Dawid Szczepankiewicz
- Ewa Pruszyńska‑Oszmałek
- Przemysław Kaczmarek
- Mathias Z. Strowski
- Krzysztof W. Nowak
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Affiliations: Department of Animal Physiology and Biochemistry, Poznań University of Life Sciences, Poznań 60‑637, Poland, Department of Hepatology and Gastroenterology and the Interdisciplinary Centre of Metabolism: Endocrinology, Diabetes and Metabolism, Charite‑University Medicine Berlin, Berlin D‑13353, Germany
Published online on: October 22, 2015 https://doi.org/10.3892/mmr.2015.4470
Pages: 8169-8175

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Abstract

Obestatin is a 23-amino acid peptide encoded by the ghrelin gene, which regulates food intake, body weight and insulin sensitivity. Obestatin influences glucose and lipid metabolism in mature adipocytes in rodents. However, the role of this peptide in rat preadipocytes remains to be fully understood. The current study characterized the effects of obestatin on lipid accumulation, preadipocyte differentiation, lipolysis and leptin secretion in rat primary preadipocytes. Obestatin enhanced lipid accumulation in rat preadipocytes and increased the expression of surrogate markers of preadipocyte differentiation. At the early stage of differentiation, obestatin suppressed lipolysis. By contrast, lipolysis was stimulated at the late stage of adipogenesis. Furthermore, obestatin stimulated the release of leptin, a key satiety hormone. Overall, the results indicated that obestatin promotes preadipocyte differentiation. Obestatin increased leptin release in preadipocytes, while the modulation of lipolysis appears to depend upon the stage of differentiation.

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