Effects of simvastatin on the osteogenic differentiation and immunomodulation of bone marrow mesenchymal stem cells

  • Authors:
    • Jianyi Niu
    • Gang Ding
    • Li Zhang
  • View Affiliations

  • Published online on: October 23, 2015     https://doi.org/10.3892/mmr.2015.4476
  • Pages: 8237-8240
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Abstract

The present study aimed to investigate the effects of simvastatin on the bone differentiation capacity and immunological characteristics of bone marrow mesenchymal stem cells (BMSCs). BMSCs were isolated and cultured in medium containing 1.0 µmol/ml simvastatin. The alkaline phosphatase activity, mRNA expression levels of osteocalcin and bone sialoprotein, and calcium nodule formation were assessed to determine the osteogenic differentiation capability of BMSCs. To investigate alterations in the immunological properties of simvastation‑treated BMSCs, the immunogenicity of these cells and the effect of BMSCs on phytohemagglutinin‑stimulated lymphocyte proliferation were also assessed. Following treatment with simvastatin, the alkaline phosphatase activity, and mRNA expression levels of osteocalcin and bone sialoprotein were increased significantly in the BMSCs. In addition, von Kossa staining revealed a brown calcium‑positive reaction zone in simvastatin‑treated cells. Simvastatin‑induced BMSCs revealed no affect on the proliferation of allogeneic lymphocytes, however, inhibited phytohemagglutinin‑induced lymphocyte proliferation. Collectively, simvastatin promoted the osteogenic differentiation of BMSCs significantly without affecting their immunosuppressive properties.
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December-2015
Volume 12 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Niu J, Ding G and Zhang L: Effects of simvastatin on the osteogenic differentiation and immunomodulation of bone marrow mesenchymal stem cells. Mol Med Rep 12: 8237-8240, 2015
APA
Niu, J., Ding, G., & Zhang, L. (2015). Effects of simvastatin on the osteogenic differentiation and immunomodulation of bone marrow mesenchymal stem cells. Molecular Medicine Reports, 12, 8237-8240. https://doi.org/10.3892/mmr.2015.4476
MLA
Niu, J., Ding, G., Zhang, L."Effects of simvastatin on the osteogenic differentiation and immunomodulation of bone marrow mesenchymal stem cells". Molecular Medicine Reports 12.6 (2015): 8237-8240.
Chicago
Niu, J., Ding, G., Zhang, L."Effects of simvastatin on the osteogenic differentiation and immunomodulation of bone marrow mesenchymal stem cells". Molecular Medicine Reports 12, no. 6 (2015): 8237-8240. https://doi.org/10.3892/mmr.2015.4476