Differential lncRNA‑mRNA co‑expression network analysis revealing the potential regulatory roles of lncRNAs in myocardial infarction

Wang,Ping; Fu,Haiying; Cui,Jiayue; Chen,Xia

doi:10.3892/mmr.2015.4669

Differential lncRNA‑mRNA co‑expression network analysis revealing the potential regulatory roles of lncRNAs in myocardial infarction

Authors:
- Ping Wang
- Haiying Fu
- Jiayue Cui
- Xia Chen
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Affiliations: Department of Otolaryngology‑Head and Neck Surgery, First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Department of Immunology, College of Basic Medical Sciences, Jilin University, Changchun, Jilin 130000, P.R. China, Department of Histology and Embryology, College of Basic Medical Sciences, Jilin University, Changchun, Jilin 130000, P.R. China, Department of Pharmacology, College of Basic Medical Sciences, Jilin University, Changchun, Jilin 130000, P.R. China
Published online on: December 10, 2015 https://doi.org/10.3892/mmr.2015.4669
Pages: 1195-1203
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Previous studies have reported that long, non‑coding RNAs (lncRNAs) are important in cardiovascular disease. However, the lncRNAs involved in myocardial infarction and their detailed mechanism have not been well characterized. In the present study, an affymetrix microarray associated with myocardial infarction was re‑annotated, following which a myocardial infarction‑related differential lncRNA‑mRNA co‑expression network (MILMN) was constructed. Subsequently, pathway enrichment analysis was used for all the mRNAs in the MILMN, and an lncRNA‑pathway network was constructed. It was found that the mRNAs were predominantly involved in certain cardiovascular disease‑associated pathway, for example the dilated cardiomyopathy and mitogen‑activated protein kinase signaling pathway. Finally, a total of 39 key lncRNAs were identified, which regulate crucial pathways in myocardial infarction. Through pathway analysis of these 39 key lncRNAs, the novel function of an annotated lncRNAs‑H19 was predicted, which may regulate apoptosis signal‑regulating kinase, which is a protein that promotes pathological cardiac remodeling following myocardial infarction. The results of the present study not only provide potential non‑coding RNA biomarkers, but also provide further insights into understanding the molecular mechanism of lncRNAs.

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