Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
May-2016 Volume 13 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
May-2016 Volume 13 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Antiadipogenic effects of subthermal electric stimulation at 448 kHz on differentiating human mesenchymal stem cells

  • Authors:
    • María Luisa Hernández‑Bule
    • Javier Martínez‑Botas
    • María Ángeles Trillo
    • Carlos L Paíno
    • Alejandro Úbeda
  • View Affiliations / Copyright

    Affiliations: Department of Bioelectromagnetics, Ramón y Cajal Health Research Institute (IRYCIS), University Hospital Ramón y Cajal, 28034 Madrid, Spain, Department of Biochemistry, Ramón y Cajal Health Research Institute (IRYCIS), University Hospital Ramón y Cajal, 28034 Madrid, Spain, Department of Neurobiology, Ramón y Cajal Health Research Institute (IRYCIS), University Hospital Ramón y Cajal, 28034 Madrid, Spain
    Copyright: © Hernández‑Bule et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3895-3903
    |
    Published online on: March 21, 2016
       https://doi.org/10.3892/mmr.2016.5032
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The 448 kHz capacitive‑resistive electric transfer (CRET) is an electrothermal therapy currently applied in anticellulite and antiobesity treatments. The aim of the present study was to determine whether exposure to the CRET electric signal at subthermal doses affected early adipogenic processes in adipose‑derived stem cells (ADSC) from human donors. ADSC were incubated for 2 or 9 days in the presence of adipogenic medium, and exposed or sham‑exposed to 5 min pulses of 448 kHz electric signal at 50 µA/mm2 during the last 48 h of the incubation. Colorimetric, immunofluorescence, western blotting and reverse transcription‑quantitative polymerase chain reaction assays were performed to assess adipogenic differentiation of the ADSC. Electric stimulation significantly decreased cytoplasmic lipid content, after both 2 and 9 days of differentiation. The antiadipogenic response in the 9 day samples was accompanied by activation of mitogen‑activated protein kinase kinase 1/2, decreased expression and partial inactivation of peroxisome proliferator‑activated receptor (PPAR) γ, which was translocated from the nucleus to the cytoplasm, together with a significant decrease in the expression levels of the PPARG1 gene, perilipin, angiopoietin‑like protein 4 and fatty acid synthase. These results demonstrated that subthermal stimulation with CRET interferes with the early adipogenic differentiation in ADSC, indicating that the electric stimulus itself can modulate processes controlling the synthesis and mobilization of fat, even in the absence of the concomitant thermal and mechanical components of the thermoelectric therapy CRET.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Hurt RT, Kulisek C, Buchanan LA and McClave SA: The obesity epidemic: Challenges, health initiatives, and implications for gastroenterologists. Gastroenterol Hepatol (NY). 6:780–792. 2010.

2 

Lavie CJ, Milani RV and Ventura HO: Obesity and cardiovascular disease: Risk factor, paradox, and impact of weight loss. J Am Coll Cardiol. 53:1925–1932. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Abraham MT and Mashkevich G: Monopolar radiofrequency skin tightening. Facial Plast Surg Clin North Am. 15:169–177. 2007. View Article : Google Scholar : PubMed/NCBI

4 

Belenky I, Margulis A, Elman M, Bar-Yosef U and Paun SD: Exploring channeling optimized radiofrequency energy: A review of radiofrequency history and applications in esthetic fields. Adv Ther. 29:249–266. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Mulholland RS, Paul MD and Chalfoun C: Noninvasive body contouring with radiofrequency, ultrasound, cryolipolysis, and low-level laser therapy. Clin Plast Surg. 38:503–520. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Alexiades-Armenakas M, Dover JS and Arndt KA: Unipolar radiofrequency treatment to improve the appearance of cellulite. J Cosmet Laser Ther. 10:148–153. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Brightman L, Weiss E, Chapas AM, Karen J, Hale E, Bernstein L and Geronemus RG: Improvement in arm and post-partum abdominal and flank subcutaneous fat deposits and skin laxity using a bipolar radiofrequency, infrared, vacuum and mechanical massage device. Lasers Surg Med. 41:791–798. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Valentim da Silva RM, Barichello PA, Medeiros ML, de Mendonça WC, Dantas JS, Ronzio OA, Froes PM and Galadari H: Effect of capacitive radiofrequency on the fibrosis of patients with cellulite. Dermatol Res Pract. 2013:7158292013.PubMed/NCBI

9 

van der Lugt C, Romero C, Ancona D, Al-Zarouni M, Perera J and Trelles MA: A multicenter study of cellulite treatment with a variable emission radio frequency system. Dermatol Ther. 22:74–84. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Boisnic S, Divaris M, Nelson AA, Gharavi NM and Lask GP: A clinical and biological evaluation of a novel, noninvasive radio-frequency device for the long-term reduction of adipose tissue. Lasers Surg Med. 46:94–103. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Hamida ZH, Comtois AS, Portmann M, Boucher JP and Savard R: Effect of electrical stimulation on lipolysis of human white adipocytes. Appl Physiol Nutr Metab. 36:271–275. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Trelles MA, van der Lugt C, Mordon S, Ribé A and Al-Zarouni M: Histological findings in adipocytes when cellulite is treated with a variable-emission radiofrequency system. Lasers Med Sci. 25:191–195. 2010. View Article : Google Scholar

13 

Kato S, Saitoh Y and Miwa N: Repressive effects of a capacitive-resistive electric transfer (CRet) hyperthermic apparatus combined with provitamin C on intracellular lipid-droplets formation in adipocytes. Int J Hyperthermia. 29:30–37. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Hernandez-Bule ML, Cid MA, Trillo MA, Leal J and Ubeda A: Cytostatic response of HepG2 to 0.57 MHz electric currents mediated by changes in cell cycle control proteins. Int J Oncol. 37:1399–1405. 2010.PubMed/NCBI

15 

Hernández-Bule ML, Paíno CL, Trillo MÁ and Úbeda A: Electric stimulation at 448 kHz promotes proliferation of human mesenchymal stem cells. Cell Physiol Biochem. 34:1741–1755. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Hernández-Bule ML, Roldán E, Matilla J, Trillo MA and Ubeda A: Radiofrequency currents exert cytotoxic effects in NB69 human neuroblastoma cells but not in peripheral blood mononuclear cells. Int J Oncol. 41:1251–1259. 2012.PubMed/NCBI

17 

Hernández-Bule ML, Trillo MA, Bazán E, Martinez-Pascual MA, Leal J and Ubeda A: Nonthermal levels of electric currents applied in capacitive electric transfer therapy provokes partial cytotoxic effects in human neuroblastoma cultures. Neurocirugia (Astur). 15:366–371. 2004. View Article : Google Scholar

18 

Hernández-Bule ML, Trillo MA, Cid MA, Leal J and Ubeda A: In vitro exposure to 0.57-MHz electric currents exerts cytostatic effects in HepG2 human hepatocarcinoma cells. Int J Oncol. 30:583–592. 2007.PubMed/NCBI

19 

Hernández-Bule ML, Trillo MÁ and Úbeda A: Molecular mechanisms underlying antiproliferative and differentiating responses of hepatocarcinoma cells to subthermal electric stimulation. PLoS One. 9:e846362014. View Article : Google Scholar : PubMed/NCBI

20 

Burgermeister E, Chuderland D, Hanoch T, Meyer M, Liscovitch M and Seger R: Interaction with MEK causes nuclear export and downregulation of peroxisome proliferator-activated receptor gamma. Mol Cell Biol. 27:803–817. 2007. View Article : Google Scholar :

21 

Burgermeister E and Seger R: MAPK kinases as nucleo-cytoplasmic shuttles for PPARgamma. Cell Cycle. 6:1539–1548. 2007. View Article : Google Scholar : PubMed/NCBI

22 

Burgermeister E and Seger R: PPARgamma and MEK interactions in cancer. PPAR Res. 2008:3094692008. View Article : Google Scholar : PubMed/NCBI

23 

Hollenberg CH and Vost A: Regulation of DNA synthesis in fat cells and stromal elements from rat adipose tissue. J Clin Invest. 47:2485–2498. 1969. View Article : Google Scholar : PubMed/NCBI

24 

Van RL, Bayliss CE and Roncari DA: Cytological and enzymological characterization of adult human adipocyte precursors in culture. J Clin Invest. 58:699–704. 1976. View Article : Google Scholar : PubMed/NCBI

25 

Zannettino AC, Paton S, Arthur A, Khor F, Itescu S, Gimble JM and Gronthos S: Multipotential human adipose-derived stromal stem cells exhibit a perivascular phenotype in vitro and in vivo. J Cell Physiol. 214:413–421. 2008. View Article : Google Scholar

26 

Pfaffl MW: A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 29:e452001. View Article : Google Scholar : PubMed/NCBI

27 

Cristancho AG and Lazar MA: Forming functional fat: A growing understanding of adipocyte differentiation. Nat Rev Mol Cell Biol. 12:722–734. 2011. View Article : Google Scholar : PubMed/NCBI

28 

Lowe CE, O'Rahilly S and Rochford JJ: Adipogenesis at a glance. J Cell Sci. 124:2681–2686. 2011. View Article : Google Scholar : PubMed/NCBI

29 

Mattijssen F and Kersten S: Regulation of triglyceride metabolism by Angiopoietin-like proteins. Biochim Biophys Acta. 1821:782–789. 2012. View Article : Google Scholar

30 

Brasaemle DL, Subramanian V, Garcia A, Marcinkiewicz A and Rothenberg A: Perilipin A and the control of triacylglycerol metabolism. Mol Cell Biochem. 326:15–21. 2009. View Article : Google Scholar : PubMed/NCBI

31 

Jayakumar A, Tai MH, Huang WY, al-Feel W, Hsu M, Abu-Elheiga L, Chirala SS and Wakil SJ: Human fatty acid synthase: Properties and molecular cloning. Proc Natl Acad Sci USA. 92:8695–8699. 1995. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Hernández‑Bule ML, Martínez‑Botas J, Trillo MÁ, Paíno CL and Úbeda A: Antiadipogenic effects of subthermal electric stimulation at 448 kHz on differentiating human mesenchymal stem cells. Mol Med Rep 13: 3895-3903, 2016.
APA
Hernández‑Bule, M.L., Martínez‑Botas, J., Trillo, M.Á., Paíno, C.L., & Úbeda, A. (2016). Antiadipogenic effects of subthermal electric stimulation at 448 kHz on differentiating human mesenchymal stem cells. Molecular Medicine Reports, 13, 3895-3903. https://doi.org/10.3892/mmr.2016.5032
MLA
Hernández‑Bule, M. L., Martínez‑Botas, J., Trillo, M. Á., Paíno, C. L., Úbeda, A."Antiadipogenic effects of subthermal electric stimulation at 448 kHz on differentiating human mesenchymal stem cells". Molecular Medicine Reports 13.5 (2016): 3895-3903.
Chicago
Hernández‑Bule, M. L., Martínez‑Botas, J., Trillo, M. Á., Paíno, C. L., Úbeda, A."Antiadipogenic effects of subthermal electric stimulation at 448 kHz on differentiating human mesenchymal stem cells". Molecular Medicine Reports 13, no. 5 (2016): 3895-3903. https://doi.org/10.3892/mmr.2016.5032
Copy and paste a formatted citation
x
Spandidos Publications style
Hernández‑Bule ML, Martínez‑Botas J, Trillo MÁ, Paíno CL and Úbeda A: Antiadipogenic effects of subthermal electric stimulation at 448 kHz on differentiating human mesenchymal stem cells. Mol Med Rep 13: 3895-3903, 2016.
APA
Hernández‑Bule, M.L., Martínez‑Botas, J., Trillo, M.Á., Paíno, C.L., & Úbeda, A. (2016). Antiadipogenic effects of subthermal electric stimulation at 448 kHz on differentiating human mesenchymal stem cells. Molecular Medicine Reports, 13, 3895-3903. https://doi.org/10.3892/mmr.2016.5032
MLA
Hernández‑Bule, M. L., Martínez‑Botas, J., Trillo, M. Á., Paíno, C. L., Úbeda, A."Antiadipogenic effects of subthermal electric stimulation at 448 kHz on differentiating human mesenchymal stem cells". Molecular Medicine Reports 13.5 (2016): 3895-3903.
Chicago
Hernández‑Bule, M. L., Martínez‑Botas, J., Trillo, M. Á., Paíno, C. L., Úbeda, A."Antiadipogenic effects of subthermal electric stimulation at 448 kHz on differentiating human mesenchymal stem cells". Molecular Medicine Reports 13, no. 5 (2016): 3895-3903. https://doi.org/10.3892/mmr.2016.5032
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team