In vitro inhibition of influenza virus infection by a crude extract from Isatis indigotica root resulting in the prevention of viral attachment

  • Authors: Zifeng Yang, Yutao Wang, Shan Zhong, Suishan Zhao, Xiangteng Zeng, Ziyao Mo, Sheng Qin, Wenda Guan, Chuyuan Li, Nanshan Zhong
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  • Published online on: Tuesday, December 13, 2011
  • Pages: 793-799
  • DOI: 10.3892/mmr.2011.709

Abstract

Isatis indigotica root (IIR) has been widely used as a Chinese medicinal herb to treat regular seasonal influenza over the long history of traditional Chinese medicinal practice. However, its inhibitory activities against influenza virus infections along with the associated mechanisms have not been investigated comprehensively. In this study, the chemical nature, mode of action and in vitro anti-influenza activities of a crude extract (G2) of IIR were characterized. The extract was found to inhibit different subtypes of human or avian influenza viruses at various magnitudes of activity (IC50 0.39‑4.3 mg/ml) in vitro, including A/PR/8/34 (H1N1), A/FM/1/47 (H1N1), A/Aichi/2/68 (H3N2), seasonal influenza (A/Guangzhou/GIRD/02/09 H1N1, B/Guangzhou/GIRD/08/09), novel swine-originating influenza (A/Guangzhou/GIRD/07/09, H1N1), A/Duck/Guangdong/09 (H6N2), A/Duck/Guangdong/94 (H7N3) and A/Chicken/Guangdong/96 (H9N2), while G2 was inactive against respiratory syncytial virus (RSV), adenovirus 3 (ADV3), parainfluenza virus 3 (PIV3) and enterovirus 71 (EV71). An apparent virus titer reduction was detected when the influenza viruses were pretreated with G2, and it was also shown that G2 exhibited inhibitory effects on influenza virus hemagglutination. In addition, G2 played a role in the early stages of infection, which did not easily result in the emergence of virus drug resistance. Thus, G2 may affect the attachment of influenza virus by interfering with the viral particles, thereby preventing the binding of influenza virus to the host cell surface.
Journal Cover

March 2012
Volume 5 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

2013 Impact Factor: 1.484
Ranked #59/122 Medicine Research and Experimental
(total number of cites)

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APA
Yang, Z., Wang, Y., Zhong, S., Zhao, S., Zeng, X., Mo, Z., Qin, S., Guan, W., Li, C., & Zhong, N. (2012). In vitro inhibition of influenza virus infection by a crude extract from Isatis indigotica root resulting in the prevention of viral attachment. Molecular Medicine Reports, 5(3), 793-799.
MLA
Yang, Wang, Zhong, Zhao, Zeng, Mo, Qin, Guan, Li, and Nanshan Zhong. "In vitro inhibition of influenza virus infection by a crude extract from Isatis indigotica root resulting in the prevention of viral attachment." Molecular Medicine Reports Molecular Medicine Reports 5.3 (2012): 793-799.
Chicago
Yang, Wang, Zhong, Zhao, Zeng, Mo, Qin, Guan, Li, and Nanshan Zhong. "In vitro inhibition of influenza virus infection by a crude extract from Isatis indigotica root resulting in the prevention of viral attachment." Molecular Medicine Reports Molecular Medicine Reports 5 no. 3 (2012): 793-799.