Microvascular density and endothelial area correlate with Ki-67 proliferative index in surgically-treated pancreatic ductal adenocarcinoma patients

Ammendola,Michele; Sacco,Rosario; Marech,Ilaria; Sammarco,Giuseppe; Zuccalà,Valeria; Luposella,Maria; Patruno,Rosa; Giordano,Marcella; Ruggieri,Eustachio; Zizzo,Nicola; Gadaleta,Cosmo  Damiano; Ranieri,Girolamo

doi:10.3892/ol.2015.3286

Microvascular density and endothelial area correlate with Ki-67 proliferative index in surgically-treated pancreatic ductal adenocarcinoma patients

Authors:
- Michele Ammendola
- Rosario Sacco
- Ilaria Marech
- Giuseppe Sammarco
- Valeria Zuccalà
- Maria Luposella
- Rosa Patruno
- Marcella Giordano
- Eustachio Ruggieri
- Nicola Zizzo
- Cosmo Damiano Gadaleta
- Girolamo Ranieri
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Affiliations: Department of Medical and Surgery Science, Clinical Surgery Unit, University of Catanzaro ‘Magna Graecia’ Medical School, Catanzaro 88100, Italy, Diagnostic and Interventional Radiology Unit with Integrated Section of Translational Medical Oncology, National Cancer Research Centre, ‘Giovanni Paolo II’, Bari 70124, Italy, Department of Health Science, Pathology Unit, University of Catanzaro ‘Magna Graecia’ Medical School, Catanzaro 88100, Italy, Department of Medical and Surgery Science, Cardiovascular Disease Unit, University of Catanzaro ‘Magna Graecia’ Medical School, Catanzaro 88100, Italy, Surgery Unit, National Cancer Research Centre, ‘Giovanni Paolo II’, Bari 70124, Italy, Chair of Pathology, ‘Aldo Moro’ University of Bari, Bari 70010, Italy
Published online on: May 27, 2015 https://doi.org/10.3892/ol.2015.3286
Pages: 967-971

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Abstract

Previous experimental and clinical data have indicated that tumour cell proliferation is associated with angiogenesis; in addition, an increased microvascular density (MVD) of tumours has been associated with poor prognosis in solid and haematological malignancies. However, limited data exists regarding the association between tumour cell proliferation and angiogenesis in primary tumour tissue from pancreatic ductal adenocarcinoma (PDAC) patients; therefore, the present study aimed to investigate this association. A series of 31 PDAC patients with stage Tumour (T)2‑3 Node (N)0‑1 Metastasis (M)0 were recruited into the present study and subsequently underwent surgery. PDAC tissue and adjacent normal tissue (ANT), resected during surgery, were evaluated using immunohistochemistry and image analysis methods to determine MVD, endothelial area (EA) and Ki‑67 expression, which is an indicator of cell proliferation rate. The results demonstrated a correlation between the above parameters with each other as well as the main clinico‑pathological features of PDAC. Significant differences were identified in MVD, EA and Ki‑67 proliferation index between PDAC and ANT. It was demonstrated that MVD, EA and Ki‑67 proliferation index were significantly correlated with each other in tumour tissue (r=0.69‑0.81; P=0.001‑0.003). However, no other significant correlations were identified. These data therefore suggested that angiogenesis and cell proliferation rate were significantly increased in PDAC compared with ANT, which provides a biological basis for the potential use of novel combinations of angiogenesis inhibitors and anti-proliferative chemotherapeutic drugs in the treatment of PDAC.

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