The effect of two nucleoside antitumor drugs on the proliferation and DNA methylation of human gastric cancer cells

Chen,Xiu‑Li; Wang,Feng‑Mei; Li,Jia‑Jia; He,Xiao‑Ying; Liu,Xi‑Yu; Ma,Li‑Bing

doi:10.3892/ol.2015.3427

The effect of two nucleoside antitumor drugs on the proliferation and DNA methylation of human gastric cancer cells

Authors:
- Xiu‑Li Chen
- Feng‑Mei Wang
- Jia‑Jia Li
- Xiao‑Ying He
- Xi‑Yu Liu
- Li‑Bing Ma
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Affiliations: School of Mathematics, Physics and Biological Engineering, Inner Mongolia University of Science and Technology, Baotou, Inner Mongolia 014010, P.R. China, School of Farm and Garden Engineering, Baotou Light Industry Vocational Technical College, Baotou, Inner Mongolia 014030, P.R. China
Published online on: June 26, 2015 https://doi.org/10.3892/ol.2015.3427
Pages: 1919-1923

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Abstract

Fluorouracil (5-Fu) and 5-azacitidine (5-aza) are two types of nucleoside analog, which have been widely applied in the treatment of several types of cancer. However, the effect of these two types of drug on the proliferation and DNA methylation of cancer cells has not been compared in a single study. In the present study, in vitro cultured human gastric cancer cells (hGCCs) were treated with various concentrations of 5‑Fu and 5‑aza, and cell counting, MTT assay and methyl‑sensitive amplified polymorphism were used to evaluate the resulting levels of proliferation and DNA methylation of hGCCs. The results revealed that the two drugs were able to inhibit the proliferation of hGCCs, but that the effect of 5‑aza was weaker than that of 5‑Fu. However, 5‑aza decreased the level of DNA methylation in hGCCs, whereas 5‑Fu did not alter DNA methylation. These results indicated that 5‑Fu was able to more efficiently inhibit the proliferation of hGCCs than 5‑aza, and that this difference may be due to differences in the anticancer mechanism of these two types of drug.

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