Differential expression of 2IgB7‑H3 and 4IgB7‑H3 in cancer cell lines and glioma tissues

Wang,Zhenxin; Yang,Jianfeng; Zhu,Yanbo; Zhu,Yun; Zhang,Bin; Zhou,Yinghui

doi:10.3892/ol.2015.3611

Differential expression of 2IgB7‑H3 and 4IgB7‑H3 in cancer cell lines and glioma tissues

Authors:
- Zhenxin Wang
- Jianfeng Yang
- Yanbo Zhu
- Yun Zhu
- Bin Zhang
- Yinghui Zhou
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Affiliations: Department of Oncology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China, Cyrus Tang Hematology Center, Soochow University, Suzhou, Jiangsu 215123, P.R. China, Department of Cerebral Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China, Department of Biochemistry and Molecular Biochemistry, School of Medicine, Soochow University, Suzhou, Jiangsu 215123, P.R. China
Published online on: August 14, 2015 https://doi.org/10.3892/ol.2015.3611
Pages: 2204-2208
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

B7‑H3 protein is an important tumor antigen, but the expression of its isoforms, 4IgB7‑H3 and 2IgB7‑H3, in tumor tissues remains unknown due to the lack of specific monoclonal antibodies (mAbs). In the present study, a mAb (9C3) specifically recognizing 2IgB7‑H3, but not 4IgB7‑H3, was prepared. Using 9C3 and a previously prepared mAb (4H7) that recognizes 4IgB7‑H3 and 2IgB7‑H3, the differential expression of 2IgB7‑H3 and 4IgB7‑H3 was analyzed in a variety of tumor cell lines by flow cytometry. It was found that 4IgB7‑H3 had a more broad spectrum of expression among the cell lines compared with 2IgB7‑H3. The expression of the two isoforms was further examined in glioma tissues using reverse transcription‑polymerase chain reaction and immunohistochemistry techniques. The data revealed that 2IgB7‑H3, but not 4IgB7‑H3, was specifically expressed in glioma. Taken together, these results demonstrated for the first time that 2IgB7‑H3 is a valuable biomarker for the diagnosis of glioma.

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1	Chapoval AI, Ni J, Lau JS, Wilcox RA, Flies DB, Liu D, Dong H, Sica GL, Zhu G, Tamada K and Chen L: B7-H3: A costimulatory molecule for T cell activation and IFN-gamma production. Nat Immunol. 2:269–274. 2001. View Article : Google Scholar : PubMed/NCBI
2	Steinberger P, Majdic O, Derdak SV, Pfistershammer K, Kirchberger S, Klauser C, Zlabinger G, Pickl WF, Stöckl J and Knapp W: Molecular characterization of human 4Ig-B7-H3, a member of the B7 family with four Ig-like domains. J Immunol. 172:2352–2359. 2004. View Article : Google Scholar : PubMed/NCBI
3	Greenwald RJ, Freeman GJ and Sharpe AH: The B7 family revisited. Annu Rev Immunol. 23:515–548. 2005. View Article : Google Scholar : PubMed/NCBI
4	Nygren MK, Tekle C, Ingebrigtsen VA, Mäkelä R, Krohn M, Aure MR, Nunes-Xavier CE, Perälä M, Tramm T, Alsner J, et al: Identifying microRNAs regulating B7-H3 in breast cancer: The clinical impact of microRNA-29c. Br J Cancer. 110:2072–2080. 2014. View Article : Google Scholar : PubMed/NCBI
5	Sun J, Mao Y, Zhang YQ, Guo YD, Mu CY, Fu FQ and Zhang XG: Clinical significance of the induction of macrophage differentiation by the costimulatory molecule B7-H3 in human non-small cell lung cancer. Oncol Lett. 6:1253–1260. 2013.PubMed/NCBI
6	Crispen PL, Sheinin Y, Roth TJ, Lohse CM, Kuntz SM, Frigola X, Thompson RH, Boorjian SA, Dong H, Leibovich BC, et al: Tumor cell and tumor vasculature expression of B7-H3 predict survival in clear cell renal cell carcinoma. Clin Cancer Res. 14:5150–5157. 2008. View Article : Google Scholar : PubMed/NCBI
7	Boland JM, Kwon ED, Harrington SM, Wampfler JA, Tang H, Yang P and Aubry MC: Tumor B7-H1 and B7-H3 expression in squamous cell carcinoma of the lung. Clin Lung Cancer. 14:157–163. 2013. View Article : Google Scholar : PubMed/NCBI
8	Ingebrigtsen VA, Boye K, Tekle C, Nesland JM, Flatmark K and Fodstad O: B7-H3 expression in colorectal cancer: Nuclear localization strongly predicts poor outcome in colon cancer. Int J Cancer. 131:2528–2536. 2012. View Article : Google Scholar : PubMed/NCBI
9	Tekle C, Nygren MK, Chen YW, Dybsjord I, Nesland JM, Maelandsmo GM and Fodstad O: B7-H3 contributes to the metastatic capacity of melanoma cells by modulation of known metastasis-associated genes. Int J Cancer. 130:2282–2290. 2012. View Article : Google Scholar : PubMed/NCBI
10	Castriconi R, Dondero A, Augugliaro R, Cantoni C, Carnemolla B, Sementa AR, Negri F, Conte R, Corrias MV, Moretta L, et al: Identification of 4Ig-B7-H3 as a neuroblastoma-associated molecule that exerts a protective role from an NK cell-mediated lysis. Proc Natl Acad Sci USA. 101:12640–12645. 2004. View Article : Google Scholar : PubMed/NCBI
11	Imai K, Wilson BS, Bigotti A, Natali PG and Ferrone S: A 94,000-dalton glycoprotein expressed by human melanoma and carcinoma cells. J Natl Cancer Inst. 68:761–769. 1982.PubMed/NCBI
12	Chen YW, Tekle C and Fodstad O: The immunoregulatory protein human B7H3 is a tumor-associated antigen that regulates tumor cell migration and invasion. Curr Cancer Drug Targets. 8:404–413. 2008. View Article : Google Scholar : PubMed/NCBI
13	Fauci JM, Sabbatino F, Wang Y, Londoño-Joshi AI, Straughn JM Jr, Landen CN, Ferrone S and Buchsbaum DJ: Monoclonal antibody-based immunotherapy of ovarian cancer: Targeting ovarian cancer cells with the B7-H3-specific mAb 376.96. Gynecol Oncol. 132:203–210. 2014. View Article : Google Scholar : PubMed/NCBI
14	Roth TJ, Sheinin Y, Lohse CM, Kuntz SM, Frigola X, Inman BA, Krambeck AE, McKenney ME, Karnes RJ, Blute ML, et al: B7-H3 ligand expression by prostate cancer: A novel marker of prognosis and potential target for therapy. Cancer Res. 67:7893–7900. 2007. View Article : Google Scholar : PubMed/NCBI
15	Wu CP, Jiang JT, Tan M, Zhu YB, Ji M, Xu KF, Zhao JM, Zhang GB and Zhang XG: Relationship between co-stimulatory molecule B7-H3 expression and gastric carcinoma histology and prognosis. World J Gastroenterol. 12:457–459. 2006.PubMed/NCBI
16	Zhou YH, Chen YJ, Ma ZY, Xu L, Wang Q, Zhang GB, Xie F, Ge Y, Wang XF and Zhang XG: 4IgB7-H3 is the major isoform expressed on immunocytes as well as malignant cells. Tissue Antigens. 70:96–104. 2007. View Article : Google Scholar : PubMed/NCBI
17	Louis DN, Ohgaki H, Wiestler OD, et al: The 2007 WHO classification of tumours of the central nervous system. Acta Neuropathol. 114:97–109. 2007. View Article : Google Scholar : PubMed/NCBI
18	Zhang G, Hou J, Shi J, Yu G, Lu B and Zhang X: Soluble CD276 (B7-H3) is released from monocytes, dendritic cells and activated T cells and is detectable in normal human serum. Immunology. 123:538–546. 2008. View Article : Google Scholar : PubMed/NCBI