Effect of BMAP‑28 on human thyroid cancer TT cells is mediated by inducing apoptosis

  • Authors:
    • Daqi Zhang
    • Lanlan Wan
    • Jinnan Zhang
    • Chang Liu
    • Hui Sun
  • View Affiliations

  • Published online on: August 14, 2015     https://doi.org/10.3892/ol.2015.3612
  • Pages: 2620-2626
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Thyroid cancer is the most common malignant endocrine tumor, with significant morbidity and mortality. Bovine myeloid antimicrobial peptide 28 (BMAP‑28) is a cathelicidin that is found in bovine neutrophils. In the present study, the effect and relative mechanism of BMAP‑28 on the human thyroid cancer TT cell line in vitro and in vivo were investigated. A 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide assay, flow cytometry and a TT‑xenograft mouse model were used in this study. The data obtained indicated that BMAP‑28 significantly inhibited the proliferation of the TT cells in vitro. In addition, the Annexin V‑fluorescein isothiocyanate/propidium iodide assay detected that BMAP‑28 induced apoptotic effects in the TT cells. Moreover, the expression of activated caspase‑3 and ‑9 was upregulated at the transcriptional and translational levels. Simultaneously, the expression of matrix metalloproteinase (MMP)3 and MMP9 was downregulated following BMAP‑28 treatment. Finally, BMAP‑28 significantly prevented the tumor growth in the TT‑xenograft mouse model. These results indicated that BMAP‑28 could be a potential agent for the treatment of thyroid cancer.
View Figures
View References

Related Articles

Journal Cover

October-2015
Volume 10 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhang D, Wan L, Zhang J, Liu C and Sun H: Effect of BMAP‑28 on human thyroid cancer TT cells is mediated by inducing apoptosis. Oncol Lett 10: 2620-2626, 2015
APA
Zhang, D., Wan, L., Zhang, J., Liu, C., & Sun, H. (2015). Effect of BMAP‑28 on human thyroid cancer TT cells is mediated by inducing apoptosis. Oncology Letters, 10, 2620-2626. https://doi.org/10.3892/ol.2015.3612
MLA
Zhang, D., Wan, L., Zhang, J., Liu, C., Sun, H."Effect of BMAP‑28 on human thyroid cancer TT cells is mediated by inducing apoptosis". Oncology Letters 10.4 (2015): 2620-2626.
Chicago
Zhang, D., Wan, L., Zhang, J., Liu, C., Sun, H."Effect of BMAP‑28 on human thyroid cancer TT cells is mediated by inducing apoptosis". Oncology Letters 10, no. 4 (2015): 2620-2626. https://doi.org/10.3892/ol.2015.3612