Serum miRNA expression in patients with esophageal squamous cell carcinoma

Hui,Beina; Chen,Xin; Hui,Lingyun; Xi,Ruxing; Zhang,Xiaozhi

doi:10.3892/ol.2015.3642

Serum miRNA expression in patients with esophageal squamous cell carcinoma

Authors:
- Beina Hui
- Xin Chen
- Lingyun Hui
- Ruxing Xi
- Xiaozhi Zhang
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Affiliations: Department of Radiation Oncology, First Affiliated Hospital of Xi'an Jiaotong University School of Medicine, Xi'an, Shaanxi 710061, P.R. China, Department of Clinical Laboratory, First Affiliated Hospital of Xi'an Jiaotong University School of Medicine, Xi'an, Shaanxi 710061, P.R. China
Published online on: August 25, 2015 https://doi.org/10.3892/ol.2015.3642
Pages: 3008-3012

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Abstract

The aim of the present study was to investigate the feasibility and value of serum microRNAs (miRNAs/miRs) as biological markers for the prediction of the behavior and prognosis of esophageal squamous cell cancer (ESCC). The differential expression of serum miRNA was detected by an miRNA microarray of 9 patients with ESCC and 9 healthy volunteers. The result of the miRNA microarray was validated in serum samples of 69 patients with ESCC and 14 healthy volunteers by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). The association between serum miRNA expression and tumor‑node‑metastasis (TNM) stage was analyzed. A total of 10 serum‑specific miRNAs were identified from the patients with ESCC. Through PCR verification, the expression levels of miR‑129, miR‑451 and miR‑365 were consistent with the microarray results validated by RT‑qPCR, and the difference was statistically significant compared with the healthy volunteers (P=0.007, P=0.007 and P<0.001, respectively). Multivariate logistic regression analysis showed that miR‑365 could serve as potential diagnostic marker for ESCC; the area under the receiver operating characteristic curve was 0.831, with a sensitivity of 80.56% and a specificity of 86.7%, but its expression did not differ significantly among the different TNM stages (stage I‑II vs. III, P=0.052; stage III vs. IV, P=0.069). The expression level of miRNA‑129 differed significantly among the different stages (stage I‑II vs. III, , P=0.002; stage III vs. IV, P=0.042), while the expression level of miR‑451 did not differ significantly between stage III and IV (P=0.308). In conclusion, serum microRNAs are novel biomarkers for ESCC, and miRNA‑365 and miRNA‑129 can be used for the early prediction of cancer and the prediction of clinical stage, respectively.

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