Overexpression of MCPH1 inhibits uncontrolled cell growth by promoting cell apoptosis and arresting the cell cycle in S and G2/M phase in lung cancer cells

Zhou,Limin; Bai,Yanjie; Li,Yanxi; Liu,Xueliang; Tan,Tao; Meng,Shasha; He,Wenting; Wu,Xiaobin; Dong,Zhifang

doi:10.3892/ol.2015.3857

Overexpression of MCPH1 inhibits uncontrolled cell growth by promoting cell apoptosis and arresting the cell cycle in S and G2/M phase in lung cancer cells

Authors:
- Limin Zhou
- Yanjie Bai
- Yanxi Li
- Xueliang Liu
- Tao Tan
- Shasha Meng
- Wenting He
- Xiaobin Wu
- Zhifang Dong
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Affiliations: Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing 400014, P.R. China, Department of Dermatology, The Chongqing Hospital of Traditional Chinese Medicine, Chongqing 400021, P.R. China, Department of Otolaryngology, The Chongqing Hospital of Traditional Chinese Medicine, Chongqing 400021, P.R. China
Published online on: November 4, 2015 https://doi.org/10.3892/ol.2015.3857
Pages: 365-372

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Abstract

Microcephalin (MCPH1/BRIT1) is a large nuclear protein that is involved in the early cellular response to DNA damage, the expression of which is reduced in a variety of types of human tumors. A recent study by our group demonstrated that MCPH1 expression is markedly decreased in lung cancer. However, it remains unclear whether inducing the expression of MCPH1 may ameliorate lung cancer, and, if so, which mechanisms underlie this process. The results of the present study demonstrated that MCPH1 expression was downregulated in lung cancer tissues compared with that in normal lung tissues. Furthermore, MCPH1 overexpression in A549 non‑small cell lung carcinoma cells, successfully inhibited cell proliferation via arrest of the cell cycle in the S and G2/M phases. In addition, MCPH1 overexpression promoted cell apoptosis, in association with a significant increase in the quantities of Bax and active caspase‑3, as well as a decrease in the level of Bcl‑2. In conclusion the present results indicated that MCPH1 is involved in the regulation of apoptosis and entry into mitosis, suggesting that MCPH1 may function as a tumor suppressor and that it may be important in the pathogenesis of lung cancer.

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