Induction of apoptosis in HCT-116 colon cancer cells by polysaccharide of Larimichthys crocea swim bladder

Suo,Huayi; Song,Jia-Le; Zhou,Yalin; Liu,Zhenhu; Yi,Ruokun; Zhu,Kai; Xie,Jie; Zhao,Xin

doi:10.3892/ol.2014.2756

Induction of apoptosis in HCT-116 colon cancer cells by polysaccharide of Larimichthys crocea swim bladder

Authors:
- Huayi Suo
- Jia-Le Song
- Yalin Zhou
- Zhenhu Liu
- Ruokun Yi
- Kai Zhu
- Jie Xie
- Xin Zhao
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Affiliations: College of Food Science, Southwest University, Chongqing 400715, P.R. China, Department of Nutrition and Food Hygiene, School of Public Health, Guilin Medical University, Guilin, Guangxi 541004, P.R. China, Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China, Science and Technology Administration, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China, Department of Food Science and Nutrition, Pusan National University, Busan 609735, Republic of Korea, Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China
Published online on: December 2, 2014 https://doi.org/10.3892/ol.2014.2756
Pages: 972-978

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Abstract

Larimichthys crocea swim bladder is a traditional food and medicine widely used in China. The in vitro anticancer effects of polysaccharide of L. crocea swim bladder (PLCSB) in HCT‑116 human colon cancer cells was investigated by 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide assay. At concentrations ranging between 0 and 800 µg/ml PLCSB, cancer cell viability was decreased by PLCSB in a concentration‑dependent manner. In particular, 400 µg/ml PLCSB significantly (P<0.05) induced apoptosis, which was demonstrated by 4,6‑diamidino‑2‑phenylindole staining and flow cytometry analysis. To elucidate the mechanisms underlying the anticancer effect of PLCSB in HCT‑116 cancer cells, the expression of apoptosis and metastasis‑associated genes was analyzed by reverse transcription‑polymerase chain reaction and western blot analysis. A total of 400 µg/ml PLCSB significantly induced apoptosis in HCT‑116 cells (P<0.05) via the upregulation Bax, p53, p21, apoptotic protease activating factor 1, caspase‑3, ‑8, and ‑9, as well as Fas and the downregulation of B‑cell lymphoma 2 (Bcl‑2), Bcl‑extra large and Fas ligand (L). The results of this study demonstrated that PLCSB exhibits an anticancer effect on HCT‑116 colon cancer cells, in vitro.

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