Role of Numb expression and nuclear translocation in endometrial cancer

Wang,Chao; Cui,Tao; Feng,Weiwei; Li,Huashun; Hu,Lina

doi:10.3892/ol.2015.2901

Role of Numb expression and nuclear translocation in endometrial cancer

Authors:
- Chao Wang
- Tao Cui
- Weiwei Feng
- Huashun Li
- Lina Hu
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Affiliations: Department of Obstetrics and Gynecology, Hospital of Fudan University, Shanghai, P.R. China, Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu, Sichuan, P.R. China, Developmental and Stem Cell Institute, West China Second Hospital, Sichuan University, Chengdu, Sichuan, P.R. China
Published online on: January 27, 2015 https://doi.org/10.3892/ol.2015.2901
Pages: 1531-1536
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The cell fate determinant Numb orchestrates numerous cell physiological and pathological processes and previous evidence has indicated that Numb expression is associated with tumorigenesis. The present study investigated the potential role of Numb in endometrial cancer (EC). Numb expression was compared between the normal endometrium and EC tissue by immunohistochemistry, and the protein levels were assessed by western blotting and confocal microscopy in the human endometrial HEC‑1B cancer cell line and normal epithelial cells. The intracellular localization of Numb in HEC‑1B cells was examined by immunofluorescence. Numb was found to be expressed at higher levels in endometrial cancer compared with the normal endometrium. Although Numb localizes to the cytoplasm and plasma membrane in the normal epithelium, the present study found that Numb accumulated in the nucleus of HEC‑1B cells. The present data reveals the upregulation of Numb expression in EC tissues and indicates that Numb plays a role in the occurrence of EC, which may be mediated by its translocation into the nucleus. The role of Numb in cancer development requires additional investigation.

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