Directional migration of adult hematopoeitic progenitors to C6 glioma in vitro

Bryukhovetskiy,Igor  Stepanovich; Mischenko,Polina   Viktorovna; Tolok,Elena  Vadimovna; Zaitcev,Sergei   Victorovich; Khotimchenko,Yuri  Stepanovich; Bryukhovetskiy,Andrei  Stepanovich

doi:10.3892/ol.2015.2952

Directional migration of adult hematopoeitic progenitors to C6 glioma in vitro

Authors:
- Igor Stepanovich Bryukhovetskiy
- Polina Viktorovna Mischenko
- Elena Vadimovna Tolok
- Sergei Victorovich Zaitcev
- Yuri Stepanovich Khotimchenko
- Andrei Stepanovich Bryukhovetskiy
View Affiliations

Affiliations: Laboratory of Molecular and Cellular Neurobiology, School of Biomedicine, Far Eastern Federal University, Vladivostok 690091, Russia
Published online on: February 10, 2015 https://doi.org/10.3892/ol.2015.2952
Pages: 1839-1844

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Multiform glioblastoma is the most common primary, highly invasive, malignant tumor of the central nervous system, with an extremely poor prognosis. The median survival of patients following surgical resection, radiation therapy and chemotherapy does not exceed 12‑15 months and thus, novel approaches for the treatment of the disease are required. The phenomenon of the directed migration of stem cells in tumor tissue presents a novel approach for the development of technologies that facilitate the targeted delivery of drugs and other therapeutic agents to the tumor foci. Hematopoietic cluster of differentiation (CD)34+/CD133+ stem cells possess significant reparative potential and are inert with respect to normal neural tissue. The aim of the present study was to investigate the substantiation ability of adult hematopoietic progenitors to the directed migration of glioma cells. A C6 glioma cell line, a culture of hematopoietic CD34+/CD133+ stem cells and primary cultures of rat astrocytes and fibroblasts were used. The cells were co‑cultured for five days. The results revealed the formation of cell shaft hematopoietic stem cells on the perimeter of the culture inserts containing the glioma culture. However, this was not observed in the wells with fibroblast and astrocyte cultures. The results indicated that hematopoietic stem cells exhibit a high potential for the directional migration of C6 glioma cells, which allows them to be considered as a promising cell line for the development of novel anticancer biomedical technologies and increases our understanding with regard to previously unclear aspects of glial tumor carcinogenesis.

View Figures

View References

1	Schiff D and Purow B: Neuro-oncology: Five new things. Neurol Clin Pract. 3:326–333. 2013. View Article : Google Scholar : PubMed/NCBI
2	Omuro A and DeAngelis LM: Glioblastoma and other malignant gliomas: a clinical review. JAMA. 310:1842–1850. 2013. View Article : Google Scholar : PubMed/NCBI
3	Yabroff KR, Harlan L, Zeruto C, Abrams J and Mann B: Patterns of care and survival for patients with glioblastoma multiforme diagnosed during 2006. Neuro Oncol. 14:351–359. PubMed/NCBI
4	Hayashi Y, Nakada M, Kinoshita M and Hamada J: Surgical strategies for nonenhancing slow-growing gliomas with special reference to functional reorganization: review with own experience. Neurol Med Chir (Tokyo). 53:438–446. 2013. View Article : Google Scholar
5	Aboody KS, Brown A, Rainov NG, Bower KA, Liu S, Yang W, Small JE, Herrlinger U, Ourednik V, Black PM, et al: Neural stem cells display extensive tropism for pathology in adult brain: evidence from intracranial gliomas. Proc Natl Acad Sci USA. 97:12846–12851. 2000. View Article : Google Scholar : PubMed/NCBI
6	Zhao D, Najbauer J, Annala AJ, Garcia E, Metz MZ, Gutova M, Polewski MD, Gilchrist M, Glackin CA, Kim SU and Aboody KS: Human neural stem cell tropism to metastatic breast cancer. Stem Cells. 30:314–325. 2012. View Article : Google Scholar
7	Gutova M, Shackleford GM, Khankaldyyan V, Herrmann KA, Shi XH, Mittelholtz K, Abramyants Y, Blanchard MS, Kim SU, Annala AJ, et al: Neural stem cell-mediated CE/CPT-11 enzyme/prodrug therapy in transgenic mouse model of intracerebellar medulloblastoma. Gene Ther. 20:143–150. 2013. View Article : Google Scholar
8	Frank RT, Najbauer J and Aboody KS: Concise review: stem cells as an emerging platform for antibody therapy of cancer. Stem Cells. 28:2084–2087. 2010. View Article : Google Scholar : PubMed/NCBI
9	Tabatabai G and Weller M: Glioblastoma stem cells. Cell Tissue Res. 343:459–465. 2011. View Article : Google Scholar : PubMed/NCBI
10	Soltanian S and Matin MM: Cancer stem cells and cancer therapy. Tumour Biol. 32:425–440. 2011. View Article : Google Scholar : PubMed/NCBI
11	Holland EC, Celestino J, Dai C, Schaefer L, Sawaya RE and Fuller GN: Combined activation of Ras and Akt in neural progenitors induces glioblastoma formation in mice. Nat Genet. 25:55–57. 2000. View Article : Google Scholar : PubMed/NCBI
12	Alexiou GA, Vartholomatos G, Karamoutsios A, Batistatou A, Kyritsis AP and Voulgaris S: Circulating progenitor cells: a comparison of patients with glioblastoma or meningioma. Acta Neurol Belg. 113:7–11. 2013. View Article : Google Scholar
13	Grobben B, De Deyn PP and Slegers H: Rat C6 glioma as experimental model system for the study of glioblastoma growth and invasion. Cell Tissue Res. 310:257–270. 2002. View Article : Google Scholar : PubMed/NCBI
14	Goffart N, Kroonen J and Rogister B: Glioblastoma-initiating cells: relationship with neural stem cells and the micro-environment. Cancers (Basel). 5:1049–1071. 2013. View Article : Google Scholar
15	Zhao D, Najbauer J, Garcia E, Metz MZ, Gutova M, Glackin CA, Kim SU and Aboody KS: Neural stem cell tropism to glioma: critical role of tumor hypoxia. Mol Cancer Res. 6:1819–1829. 2008. View Article : Google Scholar : PubMed/NCBI
16	Barth RF and Kaur B: Rat brain tumor models in experimental neuro-oncology: the C6, 9L, T9, RG2, F98, BT4C, RT-2 and CNS-1 gliomas. J Neurooncol. 94:299–312. 2009. View Article : Google Scholar : PubMed/NCBI
17	Cheng M and Qin G: Progenitor cell mobilization and recruitment: SDF-1, CXCR4, α4-integrin, and c-kit. Prog Mol Biol Transl Sci. 111:243–264. 2012. View Article : Google Scholar
18	Heese O, Disko A, Zircel D, et al: Neural stem cell migration toward gliomas in vivo. Neuro Oncol. 7:476–484. 2005. View Article : Google Scholar : PubMed/NCBI
19	Bajeto A, Barbieri F, Dorcaratto A, et al: Expression of CXC chemokine receptors 1–5 and their ligands in human glioma tissues: role of CXCR4 and SDF1 in glioma cell proliferation and migration. Neurochem Int. 49:423–432. 2006. View Article : Google Scholar
20	Cheng M, Zhou J, Wu M, Boriboun C, Thorne T, Liu T, Xiang Z, Zeng Q, Tanaka T, Tang YL, et al: CXCR4-mediated bone marrow progenitor cell maintenance and mobilization a modulated by c-kit activity. Circ Res. 107:1083–1093. 2010. View Article : Google Scholar : PubMed/NCBI
21	Kang R, Zhang Q, Zeh HJ III, Lotze MT and Tang D: HMGB1 in cancer: good, bad or both? Clin Cancer Res. 19:4046–4057. 2013. View Article : Google Scholar : PubMed/NCBI
22	Yin D, Zhang Z, Gao S and Li B: The role of chemokine receptor CXCR4 and its ligand CXCL12 in the process of proliferation and migration of oral squamous cell carcinoma. Hua Xi Kou Qiang Yi Xue Za Zhi. 31:8–12. 2013.(In Chinese). PubMed/NCBI
23	Zheng H, Ying H, Yan H, Kimmelman AC, Hiller DJ, Chen AJ, Perry SR, Tonon G, Chu GC, Ding Z, et al: p53 and Pten control neural and glioma stem/progenitor cell renewal and differentiation. Nature. 455:1129–1133. 2008. View Article : Google Scholar : PubMed/NCBI
24	Greenbaum A, Hsu YM, Day RB, Schuettpelz LG, Christopher MJ, Borgerding JN, Nagasawa T and Link DC: CXCL12 in early mesenchymal progenitors is required for hematopoietic stem-cell maintenance. Nature. 495:227–230. 2013. View Article : Google Scholar : PubMed/NCBI