Relative quantitative expression of hypoxia-inducible factor-1α, -2α and -3α, and vascular endothelial growth factor A in laryngeal carcinoma

  • Authors:
    • Todor Miroslavov Popov
    • Teodora Goranova
    • Gergana Stancheva
    • Radka Kaneva
    • Tihomir Dikov
    • Ivan Chalakov
    • Julian Rangachev
    • Dimitar Konov
    • Spiridon Todorov
    • Orlin Stoyanov
    • Vanio Mitev
  • View Affiliations

  • Published online on: March 24, 2015     https://doi.org/10.3892/ol.2015.3070
  • Pages: 2879-2885
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Abstract

The aim of the present study was to determine the relative quantitative expression of hypoxia-inducible factor (HIF)-1α, -2α and -3α, and VEGF-A in laryngeal carcinoma. A total of 63 patients with carcinoma of the larynx were enrolled in the study. Total RNA was isolated from fresh, frozen normal and tumor tissues of each patient, and quantitative polymerase chain reaction was performed. HIF‑1α was upregulated in the majority of patients (44 patients; 69.84%). By contrast, only 7 (11.11%) patients from the whole group displayed HIF‑2α overexpression, while the HIF‑3α isoform was silenced in the majority of patients (48 patients, 76.19%). A small group of 5 (7.94%) patients exhibited significant overexpression of the HIF‑3α isoform. VEGF‑A expression was significantly higher (P<0.05) in patients with upregulated HIF‑1α (2.72±1.41 RQ) compared with patients without upregulated HIF‑1α (1.86±1.46 RQ). There was a moderate positive correlation between mRNA expression levels of HIF‑1α and VEGF‑A (rs=0.392; P<0.005). To the best of our knowledge, this study is first to report quantitative data with regard to the expression of all three HIF isoforms in malignant neoplasms. The findings suggest the existence of specific phenotypes of HIF expression in laryngeal carcinoma, where the HIF switch is absent.
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June-2015
Volume 9 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Popov TM, Goranova T, Stancheva G, Kaneva R, Dikov T, Chalakov I, Rangachev J, Konov D, Todorov S, Stoyanov O, Stoyanov O, et al: Relative quantitative expression of hypoxia-inducible factor-1α, -2α and -3α, and vascular endothelial growth factor A in laryngeal carcinoma. Oncol Lett 9: 2879-2885, 2015
APA
Popov, T.M., Goranova, T., Stancheva, G., Kaneva, R., Dikov, T., Chalakov, I. ... Mitev, V. (2015). Relative quantitative expression of hypoxia-inducible factor-1α, -2α and -3α, and vascular endothelial growth factor A in laryngeal carcinoma. Oncology Letters, 9, 2879-2885. https://doi.org/10.3892/ol.2015.3070
MLA
Popov, T. M., Goranova, T., Stancheva, G., Kaneva, R., Dikov, T., Chalakov, I., Rangachev, J., Konov, D., Todorov, S., Stoyanov, O., Mitev, V."Relative quantitative expression of hypoxia-inducible factor-1α, -2α and -3α, and vascular endothelial growth factor A in laryngeal carcinoma". Oncology Letters 9.6 (2015): 2879-2885.
Chicago
Popov, T. M., Goranova, T., Stancheva, G., Kaneva, R., Dikov, T., Chalakov, I., Rangachev, J., Konov, D., Todorov, S., Stoyanov, O., Mitev, V."Relative quantitative expression of hypoxia-inducible factor-1α, -2α and -3α, and vascular endothelial growth factor A in laryngeal carcinoma". Oncology Letters 9, no. 6 (2015): 2879-2885. https://doi.org/10.3892/ol.2015.3070