Tissue-specific regulation of Apaf-1 expression by p53

  • Authors:
    • Clement K. Ho
    • Jason A. Bush
    • Gang Li
  • View Affiliations

  • Published online on: Monday, September 1, 2003
  • Pages: 1139-1143
  • DOI: 10.3892/or.10.5.1139

Abstract

The p53 tumor suppressor is a transcription factor that regulates cell cycle and apoptosis in response to a variety of environmental stress conditions. Recent studies indicated that the apoptotic protease activating factor-1 (Apaf-1) is a direct transcriptional target of p53 in DNA damage-induced apoptosis. However, the dependence of Apaf-1 expression on p53 has not been shown in normal physiological conditions. To further investigate their relationship, we first examined Apaf-1 protein levels in seven organs including brain, heart, kidney, liver, lung, spleen, and testis of wild-type (p53+/+) and p53-deficent (p53−/−) mice by Western blot analysis. The heart and spleen of the p53−/− mice showed a reduced Apaf-1 protein expression compared with the p53+/+ controls, while other organs did not show significant difference. The results from Western blot analysis were further confirmed by immunohistochemistry. Semi-quantitative RT-PCR was then employed to determine the expression of Apaf-1 transcripts. Similarly, we detected a lower Apaf-1 mRNA level in the heart and spleen of p53−/− mice compared with the p53+/+ controls, supporting a transcriptional upregulation of Apaf-1 by p53. Taken together, these results demonstrate that p53 regulation of Apaf-1 expression is tissue-specific.
Journal Cover

September 2003
Volume 10 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

2013 Impact Factor: 2.191
Ranked #33/202 Oncology
(total number of cites)

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APA
Ho, C., Bush, J., & Li, G. (2003). Tissue-specific regulation of Apaf-1 expression by p53. Oncology Reports, 10(5), 1139-1143.
MLA
Ho, Bush, and Gang Li. "Tissue-specific regulation of Apaf-1 expression by p53." Oncology Reports Oncology Reports 10.5 (2003): 1139-1143.
Chicago
Ho, Bush, and Gang Li. "Tissue-specific regulation of Apaf-1 expression by p53." Oncology Reports Oncology Reports 10 no. 5 (2003): 1139-1143.