Fas stimulation activates NF-κB in SK-Hep1 hepatocellular carcinoma cells

  • Authors:
    • Hiroshi Okano
    • Katsuya Shiraki
    • Hidekazu Inoue
    • Tomoyuki Kawakita
    • Yukiko Saitou
    • Naoyuki Enokimura
    • Norihiko Yamamoto
    • Kazushi Sugimoto
    • Kazumoto Murata
    • Takeshi Nakano
  • View Affiliations

  • Published online on: Monday, September 1, 2003
  • Pages: 1145-1148
  • DOI: 10.3892/or.10.5.1145

Abstract

The TNF-receptor family has a dual signaling pathway, including induction of apoptosis and NF-κB activation associated with cell survival. Hepatocellular carcinoma (HCC) cells express TNF-receptor family members and the signaling from these receptors induces NF-κB activation. However, the role of Fas in induction of NF-κB activation in HCC cells is not well understood. In this study, SK-Hep1, HepG2 or HLE cells were stimulated by anti-Fas agonistic antibody. Fas stimulation induced NF-κB activation in a dose-dependent manner in SK-Hep1 and HepG2 cell lines, but not in HLE cells. Anti-Fas agonistic antibody or the metabolic inhibitor, cyclo-heximide (CHX), failed to kill SK-Hep1 cells, but co-incubation with anti-Fas agonistic antibody and CHX was effective for induction of apoptosis. SK-Hep1 cell lines receiving Fas stimulation had increased viability, but the extent of cell proliferation was not dose-dependent. The observation suggests that Fas stimulation may contribute to HCC cell survival or proliferation.
Journal Cover

September 2003
Volume 10 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

2013 Impact Factor: 2.191
Ranked #33/202 Oncology
(total number of cites)

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APA
Okano, H., Shiraki, K., Inoue, H., Kawakita, T., Saitou, Y., Enokimura, N., Yamamoto, N., Sugimoto, K., Murata, K., & Nakano, T. (2003). Fas stimulation activates NF-κB in SK-Hep1 hepatocellular carcinoma cells. Oncology Reports, 10(5), 1145-1148.
MLA
Okano, Shiraki, Inoue, Kawakita, Saitou, Enokimura, Yamamoto, Sugimoto, Murata, and Takeshi Nakano. "Fas stimulation activates NF-κB in SK-Hep1 hepatocellular carcinoma cells." Oncology Reports Oncology Reports 10.5 (2003): 1145-1148.
Chicago
Okano, Shiraki, Inoue, Kawakita, Saitou, Enokimura, Yamamoto, Sugimoto, Murata, and Takeshi Nakano. "Fas stimulation activates NF-κB in SK-Hep1 hepatocellular carcinoma cells." Oncology Reports Oncology Reports 10 no. 5 (2003): 1145-1148.