Mutation analysis of CTNNB1 (β-catenin) and AXIN1, the components of Wnt pathway, in cervical carcinomas

  • Authors: Tsung-Hsien Su, Jan-Gowth Chang, Kun-Tu Yeh, Ting-Hsu Lin, Tai-Ping Lee, Jui-Chang Chen, Cheng-Chieh Lin
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  • Published online on: Monday, September 1, 2003
  • Pages: 1195-1200
  • DOI: 10.3892/or.10.5.1195

Abstract

The components of the Wnt-signaling pathway are mutated in tumors, but the relationship between these components and cervical cancer has not been elucidated. In this study, we used immunohistochemistry, single strand confirmation polymorphism (SSCP) and direct sequencing methods to analyze the mutation and protein expressions of both CTNNB1 and AXIN1 in cervical cancer. Among the 30 tested cervical cancers, no mutation of CTNNB1 but 3 polymorphisms were found. Mutation analysis of AXIN1 revealed that one specimen had a heterozygous mutation at codon 740 (GCC↷ACC) and six polymorphisms were also found. Immunohistochemistry showed no relationship between the protein expression patterns and mutation of AXIN1 and CTNNB1. Mutations of CTNNB1 may not be a factor, whereas mutations of AXIN1 may play a limited role in tumorigenesis of cervical cancer. In addition, aberrant expression patterns are not mutation related, so that other factors may be responsible for these changes.
Journal Cover

September 2003
Volume 10 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

2012 Impact Factor: 2.191
Ranked #33/202 Oncology
(total number of cites)

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APA
Su, T., Chang, J., Yeh, K., Lin, T., Lee, T., Chen, J., & Lin, C. (2003). Mutation analysis of CTNNB1 (β-catenin) and AXIN1, the components of Wnt pathway, in cervical carcinomas. Oncology Reports, 10(5), 1195-1200.
MLA
Su, Chang, Yeh, Lin, Lee, Chen, and Cheng-Chieh Lin. "Mutation analysis of CTNNB1 (β-catenin) and AXIN1, the components of Wnt pathway, in cervical carcinomas." Oncology Reports Oncology Reports 10.5 (2003): 1195-1200.
Chicago
Su, Chang, Yeh, Lin, Lee, Chen, and Cheng-Chieh Lin. "Mutation analysis of CTNNB1 (β-catenin) and AXIN1, the components of Wnt pathway, in cervical carcinomas." Oncology Reports Oncology Reports 10 no. 5 (2003): 1195-1200.