Establishment and characterization of sublines of LNCaP human prostate cancer cells

  • Authors: X. Steven Wan, Zhaozong Zhou, Vernon Steele, Levy Kopelovich, Ann R. Kennedy
  • View Affiliations

  • Published online on: Monday, September 1, 2003
  • Pages: 1569-1575
  • DOI: 10.3892/or.10.5.1569

Abstract

The present study was undertaken to develop a panel of human prostate cancer cell sublines that represent a phenotypic continuum of prostate carcinogenesis. We cloned and established more than two dozen LNCaP sublines from parental LNCaP cells by the limiting dilution method, akin to a fluctuation analysis, in vitro. The newly established LNCaP sublines differ in hormone-sensitivity, anchorage-independent growth ability and rate of PSA production. These LNCaP sublines may represent the naturally occurring heterogeneity in human prostate cancer and, therefore, could be useful for studying the effects of anticarcinogenic agents on cell clones that are derived from the same parental cell population.
Journal Cover

September 2003
Volume 10 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

2013 Impact Factor: 2.191
Ranked #33/202 Oncology
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Wan, X., Zhou, Z., Steele, V., Kopelovich, L., & Kennedy, A. (2003). Establishment and characterization of sublines of LNCaP human prostate cancer cells. Oncology Reports, 10(5), 1569-1575.
MLA
Wan, Zhou, Steele, Kopelovich, and Ann Kennedy. "Establishment and characterization of sublines of LNCaP human prostate cancer cells." Oncology Reports Oncology Reports 10.5 (2003): 1569-1575.
Chicago
Wan, Zhou, Steele, Kopelovich, and Ann Kennedy. "Establishment and characterization of sublines of LNCaP human prostate cancer cells." Oncology Reports Oncology Reports 10 no. 5 (2003): 1569-1575.