Amplification/overexpression of Aurora-A in human gastric carcinoma: Potential role in differentiated type gastric carcinogenesis

  • Authors:
    • Kiyoshi Kamada
    • Yukishige Yamada
    • Tomoko Hirao
    • Heisuke Fujimoto
    • Yasushi Takahama
    • Masatoh Ueno
    • Tomoyoshi Takayama
    • Akihiko Naito
    • Shuya Hirao
    • Yoshiyuki Nakajima
  • View Affiliations

  • Published online on: September 1, 2004     https://doi.org/10.3892/or.12.3.593
  • Pages: 593-599
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Aurora-A encodes a cell cycle regulated serine/threonine kinase that has essential functions for centrosome maturation and chromosome segregation. Aurora-A is amplified and overexpressed in various human carcinomas and is suggested to be a potential oncogene. To clarify the potential role of Aurora-A in human gastric carcinoma, we examined the amplification and expression in both tumor cell lines and primary carcinoma. We examined the amplification and overexpression of Aurora-A in 9 gastric carcinoma cell lines and 88 primary gastric carcinomas using Southern and Northern blot analysis, and confirmed a protein expression by immunohistochemical staining. We also investigated the relationship between Aurora-A overexpression and clinicopathological features of the tumors. Aurora-A amplification and overexpression was observed in 29% and 44.4% of cell lines and 12.5% and 41% of primary carcinomas, respectively. There was discordance between gene amplification and transcript expression, since in a previous study DNA amplification was the main mechanism for Aurora-A activation. Aurora-A overexpression exhibited significant association with increasing age and differentiated type gastric carcinoma. It was also detected in early stage gastric cancer as well as in gastric intestinal metaplasia, which is considered as a common precursor lesion for the differentiated type gastric carcinoma, and severe dysplastic cells showed stronger protein expression. We concluded that Aurora-A overexpression may well be involved in differentiated type gastric carcinogenesis. Further evaluation of the possible roles of Aurora-A and the regulation of Aurora-A expression in malignant cells will be critically important for the development of new strategies aimed at controlling the growth of malignant cells.

Related Articles

Journal Cover

September 2004
Volume 12 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Kamada K, Yamada Y, Hirao T, Fujimoto H, Takahama Y, Ueno M, Takayama T, Naito A, Hirao S, Nakajima Y, Nakajima Y, et al: Amplification/overexpression of Aurora-A in human gastric carcinoma: Potential role in differentiated type gastric carcinogenesis. Oncol Rep 12: 593-599, 2004.
APA
Kamada, K., Yamada, Y., Hirao, T., Fujimoto, H., Takahama, Y., Ueno, M. ... Nakajima, Y. (2004). Amplification/overexpression of Aurora-A in human gastric carcinoma: Potential role in differentiated type gastric carcinogenesis. Oncology Reports, 12, 593-599. https://doi.org/10.3892/or.12.3.593
MLA
Kamada, K., Yamada, Y., Hirao, T., Fujimoto, H., Takahama, Y., Ueno, M., Takayama, T., Naito, A., Hirao, S., Nakajima, Y."Amplification/overexpression of Aurora-A in human gastric carcinoma: Potential role in differentiated type gastric carcinogenesis". Oncology Reports 12.3 (2004): 593-599.
Chicago
Kamada, K., Yamada, Y., Hirao, T., Fujimoto, H., Takahama, Y., Ueno, M., Takayama, T., Naito, A., Hirao, S., Nakajima, Y."Amplification/overexpression of Aurora-A in human gastric carcinoma: Potential role in differentiated type gastric carcinogenesis". Oncology Reports 12, no. 3 (2004): 593-599. https://doi.org/10.3892/or.12.3.593