Overexpression of constitutive signal transducer and activator of transcription 3 mRNA in cisplatin-resistant human non-small cell lung cancer cells

  • Authors: Kenji Ikuta, Kazu Takemura, Masaru Kihara, Masuhiro Nishimura, Nobuhiko Ueda, Shinsaku Naito, Eibai Lee, Eiji Shimizu, Aiko Yamauchi
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  • Published online on: Tuesday, February 1, 2005
  • Pages: 217-222
  • DOI: 10.3892/or.13.2.217

Abstract

Non-small cell lung cancer (NSCLC) often shows intrinsic multidrug resistance, which is one of the most serious problems in cisplatin-based adjuvant chemotherapy. Recently, the constitutive activation of signal transducer and activator of transcription (STAT) factors has been found in a variety of human cancers. In the present study, the mRNA expression of STATs in various human NSCLC cell lines was investigated by quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) to determine whether STATs can be implicated in cisplatin resistance and apoptosis inducibility. Cisplatin triggered apoptosis in Ma-46 based on biochemical and morphological findings, but not in Ma-31. The mRNA expression of STAT3 was highest in cisplatin-resistant Ma-31 and lowest in cisplatin-sensitive Ma-46. A 6-hour exposure of cancer cells to cisplatin failed to stimulate STAT3 mRNA expression. Therefore, an increased transcriptional level of constitutive STAT3 may be related to the suppressive regulation of the apoptotic pathway in intrinsically chemo-resistant NSCLC cells.
Journal Cover

February 2005
Volume 13 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

2013 Impact Factor: 2.191
Ranked #33/202 Oncology
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APA
Ikuta, K., Takemura, K., Kihara, M., Nishimura, M., Ueda, N., Naito, S., Lee, E., Shimizu, E., & Yamauchi, A. (2005). Overexpression of constitutive signal transducer and activator of transcription 3 mRNA in cisplatin-resistant human non-small cell lung cancer cells. Oncology Reports, 13(2), 217-222.
MLA
Ikuta, Takemura, Kihara, Nishimura, Ueda, Naito, Lee, Shimizu, and Aiko Yamauchi. "Overexpression of constitutive signal transducer and activator of transcription 3 mRNA in cisplatin-resistant human non-small cell lung cancer cells." Oncology Reports Oncology Reports 13.2 (2005): 217-222.
Chicago
Ikuta, Takemura, Kihara, Nishimura, Ueda, Naito, Lee, Shimizu, and Aiko Yamauchi. "Overexpression of constitutive signal transducer and activator of transcription 3 mRNA in cisplatin-resistant human non-small cell lung cancer cells." Oncology Reports Oncology Reports 13 no. 2 (2005): 217-222.