Effects of antineoplaston AS2-1 against post-operative lung metastasis in orthotopically implanted colon cancer in nude rat

  • Authors: Keiko Matono, Yutaka Ogata, Hideaki Tsuda, Yasumi Araki, Kazuo Shirouzu
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  • Published online on: Tuesday, March 1, 2005
  • Pages: 389-395
  • DOI: 10.3892/or.13.3.389

Abstract

We have investigated the efficacy and mechanisms of antineoplaston AS2-1 against post-operative lung metastasis following removal of implanted human colon cancer in nude rat. The influence of AS2-1 on in vitro KM12SM human colon carcinoma cell activities (growth, cell cycle, and apoptosis) was evaluated. AS2-1 was administered perorally after removal of the implanted KM12SM cecal cancer in nude rat. AS2-1 inhibited KM12SM cell proliferation through G1 cell arrest and, at a higher concentration, induction of apoptosis. AS2-1 showed significant reduction in lung metastasis at 5 weeks after cecal removal. The survival rate in the AS2-1 group was significantly higher than that in the control. TUNEL staining on the lung metastatic tumors revealed that the apoptosis index (AI) in the AS2-1 group was significantly higher. Antineoplaston AS2-1 showed an antimetastatic effect against post-operative lung metastases from colon cancer through G1 cell arrest and the subsequent induction of apoptosis.
Journal Cover

March 2005
Volume 13 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

2012 Impact Factor: 2.297
Ranked #36/196 Oncology
(total number of cites)

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APA
Matono, K., Ogata, Y., Tsuda, H., Araki, Y., & Shirouzu, K. (2005). Effects of antineoplaston AS2-1 against post-operative lung metastasis in orthotopically implanted colon cancer in nude rat. Oncology Reports, 13(3), 389-395.
MLA
Matono, Ogata, Tsuda, Araki, and Kazuo Shirouzu. "Effects of antineoplaston AS2-1 against post-operative lung metastasis in orthotopically implanted colon cancer in nude rat." Oncology Reports Oncology Reports 13.3 (2005): 389-395.
Chicago
Matono, Ogata, Tsuda, Araki, and Kazuo Shirouzu. "Effects of antineoplaston AS2-1 against post-operative lung metastasis in orthotopically implanted colon cancer in nude rat." Oncology Reports Oncology Reports 13 no. 3 (2005): 389-395.