Induction of apoptosis in human ovarian epithelial cancer cells by antisurvivin oligonucleotides

  • Authors:
    • Xiangyi Ma
    • Shixuan Wang
    • Jianfeng Zhou
    • Hui Xing
    • Gang Xu
    • Beibei Wang
    • Gang Chen
    • Yun-Ping Lu
    • Ding Ma
  • Corresponding author:
  • View Affiliations

  • Published online on: Friday, July 1, 2005
  • Pages: 275-279 DOI: 10.3892/or.14.1.275

Abstract

Survivin, an anti-apoptosis gene that is abnormally overexpressed in a variety of human tumors, may play an important role in the carcinogenesis and drug resistance of cancer. This study was designed to explore the effects of liposome-survivin antisense oligonucleotide (Lip-ASODN) on the growth and apoptosis of human ovarian cancer cell lines, A2780 and SKOV3. To investigate the use of survivin as a therapeutic target on ovarian cancer, we carried out transfections with Lip-ASODN to induce apoptosis in ovarian cancer cell lines, A2780 and SKOV3. The expression of survivin mRNA and relative protein were evaluated separately by quantitative real-time RT-PCR and Western blot analysis. Cell proliferation inhibition was determined by methyl thiazolyl tetrazolium (MTT) assay, and the induced cell apoptosis was examined using flow cytometry (FCM) after Lip-ASODN transfection. Our results showed that the overexpression of survivin led to infinite carcino-proliferation, and survivin expression in the survivin-positive ovarian cancer cell line A2780 and SKOV3 cells was significantly and gradually reduced when transfected with Lip-ASODN at concentrations of 200, 400 and 600 nM by degrees. Lip-ASODN transfection induced greater apoptosis rates in the human ovarian cancer cell lines A2780 and SKOV3 (p<0.05). The growth inhibition and apoptotic rates of tumor cells change when treated with different concentrations of Lip-ASODN. The cell growth inhibition peak rate was reached when increasing Lip-ASODN concentration to 600 nM. Furthermore, time course evaluation showed that survivin protein expression was inhibited by Lip-ASODN within 12 h after transfection. We concluded that down-regulation of survivin by a targeted antisense oligonucleotide appears to be an effective gene therapy approach in the treatment of ovarian cancer.
Journal Cover

July 2005
Volume 14 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

2013 Impact Factor: 2.191
2014 I.F. (Expected) ≥ 2.504 Ranked #33/202 Oncology
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APA
Ma, X., Wang, S., Zhou, J., Xing, H., Xu, G., Wang, B. ... Ma, D. (2005). Induction of apoptosis in human ovarian epithelial cancer cells by antisurvivin oligonucleotides. Oncology Reports, 14, 275-279. http://dx.doi.org/10.3892/or.14.1.275
MLA
Ma, X., Wang, S., Zhou, J., Xing, H., Xu, G., Wang, B., Chen, G., Lu, Y., Ma, D."Induction of apoptosis in human ovarian epithelial cancer cells by antisurvivin oligonucleotides". Oncology Reports 14.1 (2005): 275-279.
Chicago
Ma, X., Wang, S., Zhou, J., Xing, H., Xu, G., Wang, B., Chen, G., Lu, Y., Ma, D."Induction of apoptosis in human ovarian epithelial cancer cells by antisurvivin oligonucleotides". Oncology Reports 14, no. 1 (2005): 275-279. http://dx.doi.org/10.3892/or.14.1.275