Chinese medicine Angelica sinensis suppresses radiation-induced expression of TNF-α and TGF-β1 in mice

  • Authors:
    • Cong-Hua Xie
    • Ming-Sheng Zhang
    • Yun-Feng Zhou
    • Guang Han
    • Zhen Cao
    • Fu-Xiang Zhou
    • Gong Zhang
    • Zhi-Guo Luo
    • Jian-Ping Wu
    • Hui Liu
    • Ji Chen
    • Wen Jie Zhang
  • View Affiliations

  • Published online on: Thursday, June 1, 2006
  • Pages: 1429-1436
  • DOI: 10.3892/or.15.6.1429

Abstract

Radiotherapy of thoracic cancer often causes pulmonary inflammation leading to pneumonitis and fibrosis. We favor the hypothesis that cytokine-mediated multicellular interactions may result in the overexpression of proinflammatory cytokines such as TNF-α and TGF-β1, which promotes progressive radiation-induced lung injury. The root of Angelica sinensis, known as ‘Danggui’ in Chinese medicine, is widely used to treat radiation-induced pneumonitis in humans and shows clinical efficacy and low/no toxicity with an unclear mechanism. Using quantitative RT-PCR and immunohistochemistry (IHC) methods, we investigated radiation-induced lung injury in a mouse model. C57BL/6 mice were assigned to 4 groups: no treatment (NT), Angelica Sinensis treatment only (AS), X-ray irradiation only (XRT, single fraction of 12 Gy irradiation to the thoraces) and AS treatment plus XRT (AS/XRT). Mice in NT and AS groups exhibited low TNF-α and TGF-β1 mRNA levels and few positive cell counts for TNF-α (8-17 cells per field, x400 magnification) and TGF-β1 (9-31 cells per field), respectively. In XRT mice, there were increased inflammatory cells positive for TNF-α and TGF-β1 in lung tissue compared with NT mice (P<0.01). However, when XRT mice received AS treatment (AS/XRT), the number of inflammatory cells in lung tissue positive for both TNF-α and TGF-β1 was decreased compared with XRT-only mice (P<0.01) accompanied by moderately decreased mRNA levels of TNF-α and TGF-β1. We conclude that radiation induces expression of TNF-α and TGF-β1 in the inflammatory cells of irradiated lung tissue during the pneumonic phase. The predominant localization of TNF-α and TGF-β1 in inflammatory cell infiltrates suggests these cytokines' involvement in the process of radiation-induced pneumonitis. Moreover, effective down-regulation of TNF-α and TGF-β1 in irradiated lung tissue by Angelica Sinensis is, at least in part, indicative of its clinical efficacy in treating radiation-induced pneumonitis.
Journal Cover

June 2006
Volume 15 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

2013 Impact Factor: 2.191
Ranked #33/202 Oncology
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APA
Xie, C., Zhang, M., Zhou, Y., Han, G., Cao, Z., Zhou, F., Zhang, G., Luo, Z., Wu, J., Liu, H., Chen, J., & Zhang, W. (2006). Chinese medicine Angelica sinensis suppresses radiation-induced expression of TNF-α and TGF-β1 in mice. Oncology Reports, 15(6), 1429-1436.
MLA
Xie, Zhang, Zhou, Han, Cao, Zhou, Zhang, Luo, Wu, Liu, Chen, and Wen Zhang. "Chinese medicine Angelica sinensis suppresses radiation-induced expression of TNF-α and TGF-β1 in mice." Oncology Reports Oncology Reports 15.6 (2006): 1429-1436.
Chicago
Xie, Zhang, Zhou, Han, Cao, Zhou, Zhang, Luo, Wu, Liu, Chen, and Wen Zhang. "Chinese medicine Angelica sinensis suppresses radiation-induced expression of TNF-α and TGF-β1 in mice." Oncology Reports Oncology Reports 15 no. 6 (2006): 1429-1436.