Metastasis-associated protein 1 enhances angiogenesis by stabilization of HIF-1α

  • Authors: Hyo-Eun Moon, Hwanju Cheon, Kwang-Hoon Chun, Seung Ki Lee, Yong-Sik Kim, Bo-Kyung Jung, Jeong Ae Park, Se-Hee Kim, Joo-Won Jeong, Myung-Shik Lee
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  • Published online on: Sunday, October 1, 2006
  • Pages: 929-935
  • DOI: 10.3892/or.16.4.929

Abstract

Metastasis-associated protein 1 (MTA1) is highly upregulated in cancer cells with metastatic potential; however, the molecular mechanism by which MTA1 increases the metastatic potential of cancer cells is unknown. We characterized the functional consequences of MTA1 overexpression in cancer cells with an emphasis on its potential role as a deacetylator of hypoxia-inducible factor-1α (HIF-1α). MTA1 increased the expression of HIF-1α protein, but did not increase the expression of its mRNA. Glutathione S-transferase pull-down and coimmunoprecipitation assays demonstrated direct interaction of MTA1 with HIF-1α both in vitro and in vivo. Immunoprecipitation and acetylation assays also showed that MTA1 has deacetylation activity on HIF-1α in vivo. Moreover, MTA1 increased the transcriptional activity of HIF-1α and enhanced the expression of vascular endothelial growth factor, a target molecule of HIF-1α. Conditioned medium collected from MTA1 transfectants also increased angiogenesis in vitro and in vivo, probably through enhanced HIF-1α stabilization. These results indicate that MTA1 enhances angiogenesis by stabilization of the HIF-1α protein, which is closely related to the increased metastatic potential of cancer cells with high MTA1 expression.
Journal Cover

October 2006
Volume 16 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

2012 Impact Factor: 2.297
Ranked #36/196 Oncology
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APA
Moon, H., Cheon, H., Chun, K., Lee, S., Kim, Y., Jung, B., Park, J., Kim, S., Jeong, J., & Lee, M. (2006). Metastasis-associated protein 1 enhances angiogenesis by stabilization of HIF-1α. Oncology Reports, 16(4), 929-935.
MLA
Moon, Cheon, Chun, Lee, Kim, Jung, Park, Kim, Jeong, and Myung-Shik Lee. "Metastasis-associated protein 1 enhances angiogenesis by stabilization of HIF-1α." Oncology Reports Oncology Reports 16.4 (2006): 929-935.
Chicago
Moon, Cheon, Chun, Lee, Kim, Jung, Park, Kim, Jeong, and Myung-Shik Lee. "Metastasis-associated protein 1 enhances angiogenesis by stabilization of HIF-1α." Oncology Reports Oncology Reports 16 no. 4 (2006): 929-935.