Ethyl acetate extract of Chinese medicinal herb Sarcandra glabra induces growth inhibition on human leukemic HL-60 cells, associated with cell cycle arrest and up-regulation of pro-apoptotic Bax/Bcl-2 ratio

  • Authors: W. Y. Li, Lawrence C.M. Chiu, W. S. Lam, W. Y. Wong, Y. T. Chan, Y. P. Ho, Elaine Y.L. Wong, Y. S. Wong, Vincent E.C. Ooi
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  • Published online on: Thursday, February 1, 2007
  • Pages: 425-431
  • DOI: 10.3892/or.17.2.425

Abstract

Sarcandra glabra (Thunb.) Nakai, colloquially known as Caoshanhu, is a Chinese medicinal herb with reported anti-tumor, anti-inflammatory, anti-viral and non-specific immunoenhancing properties. Although the plant has been clinically used for treating a variety of diseases, its bioactive ingredients are largely unknown and its mode of action has never been investigated. In this study, the anti-tumor property of ethyl acetate (EA) extract of S. glabra was investigated by determining its in vitro growth-inhibitory effects on a panel of human cancer cell lines of different histotypes. Growth inhibition of the EA extract on the cancer cells seemed to be selective, and the leukemic HL-60 was found to be the most responsive after 48 h of treatment (IC50=58 µg/ml). Flow cytometric studies further illustrated that the extract might interfere with DNA replication and thus arrested the cell cycle at S phase in the leukemic cells, followed by DNA fragmentation and loss of phospholipid asymmetry in the plasma membrane after 72 h of treatment. Concurrently, the pro-apoptotic Bax/Bcl-2 ratio was also up-regulated by more than 178% of the control level. All these findings suggested that the extract had initiated apoptosis to kill the leukemic cells. Results from this pioneer study help to establish a scientific foundation for future research and development of the bioactive ingredients in EA extract of S. glabra as efficacious anti-cancer agents.
Journal Cover

February 2007
Volume 17 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

2013 Impact Factor: 2.191
Ranked #33/202 Oncology
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APA
Li, W., Chiu, L., Lam, W., Wong, W., Chan, Y., Ho, Y., Wong, E., Wong, Y., & Ooi, V. (2007). Ethyl acetate extract of Chinese medicinal herb Sarcandra glabra induces growth inhibition on human leukemic HL-60 cells, associated with cell cycle arrest and up-regulation of pro-apoptotic Bax/Bcl-2 ratio. Oncology Reports, 17(2), 425-431.
MLA
Li, Chiu, Lam, Wong, Chan, Ho, Wong, Wong, and Vincent Ooi. "Ethyl acetate extract of Chinese medicinal herb Sarcandra glabra induces growth inhibition on human leukemic HL-60 cells, associated with cell cycle arrest and up-regulation of pro-apoptotic Bax/Bcl-2 ratio." Oncology Reports Oncology Reports 17.2 (2007): 425-431.
Chicago
Li, Chiu, Lam, Wong, Chan, Ho, Wong, Wong, and Vincent Ooi. "Ethyl acetate extract of Chinese medicinal herb Sarcandra glabra induces growth inhibition on human leukemic HL-60 cells, associated with cell cycle arrest and up-regulation of pro-apoptotic Bax/Bcl-2 ratio." Oncology Reports Oncology Reports 17 no. 2 (2007): 425-431.