Rearrangement and expression of myeov and hst in NIH/3T3 transfectants: A caveat for the interpretation of DNA transfection analyses

  • Authors:
    • Marcus Brecht
    • Agatha C.M. Steenvoorden
    • Susanne Luf
    • Claus R. Bartram
    • Johannes W.G. Janssen
  • View Affiliations

  • Published online on: Tuesday, May 1, 2007
  • Pages: 1127-1131
  • DOI: 10.3892/or.17.5.1127

Abstract

By means of the tumorigenicity assay applying DNA from a patient with a gastric carcinoma (MA) we have already reported the identification of the putative oncogene myeov. In addition we have shown its involvement in t(11;14)-positive multiple myelomas and amplifications of breast tumours and esophageal carcinomas. The failure of myeov cDNA to induce tumour formation in NIH/3T3 cells prompted us to analyze the human sequences present in our MA-T1a1 tertiary transfectants. Sequence analysis revealed the presence of the human oncogene hst (fgf4) at a distance of ≈9kb from the myeov gene in our MA-T1a1 tertiary transfectants. Both myeov and hst (fgf4) are normally situated ≈475-kb apart at band 11q13, a region that is frequently amplified and overexpressed in various tumours. Southern and Northern blot analyses confirmed our sequence data and showed rearrangement of hst sequences during the transfection process and its expression in our MA-T1a1 tertiary transfectants.
Journal Cover

May 2007
Volume 17 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

2013 Impact Factor: 2.191
Ranked #33/202 Oncology
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APA
Brecht, M., Steenvoorden, A., Luf, S., Bartram, C., & Janssen, J. (2007). Rearrangement and expression of myeov and hst in NIH/3T3 transfectants: A caveat for the interpretation of DNA transfection analyses. Oncology Reports, 17(5), 1127-1131.
MLA
Brecht, Steenvoorden, Luf, Bartram, and Johannes Janssen. "Rearrangement and expression of myeov and hst in NIH/3T3 transfectants: A caveat for the interpretation of DNA transfection analyses." Oncology Reports Oncology Reports 17.5 (2007): 1127-1131.
Chicago
Brecht, Steenvoorden, Luf, Bartram, and Johannes Janssen. "Rearrangement and expression of myeov and hst in NIH/3T3 transfectants: A caveat for the interpretation of DNA transfection analyses." Oncology Reports Oncology Reports 17 no. 5 (2007): 1127-1131.