GADD34 induces p21 expression and cellular senescence

  • Authors: Kahori Minami, Hirokazu Inoue, Takao Terashita, Takahiro Kawakami, Ryosuke Watanabe, Masataka Haneda, Ken-Ichi Isobe, Hidetoshi Okabe, Tokuhiro Chano
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  • Published online on: Friday, June 1, 2007
  • Pages: 1481-1485
  • DOI: 10.3892/or.17.6.1481

Abstract

We previously identified GADD34 (growth arrest and DNA damage protein 34) by screening for genes involved in oncogenic-transformation and/or cellular senescence in Ras-transformed rat F2408 fibroblasts (7EJ-Ras), which exhibit anchorage-independent growth and do not senesce. In the current study, we found that transduction of 7EJ-Ras cells with a retroviral vector expressing GADD34 suppressed their proliferation. Furthermore, we observed that fibroblasts derived from GADD34-knockout mice (GADD34-KO MEFs) did not undergo senescence. Whereas the expression of p21 was decreased in GADD34 KO MEFs, its expression was rescued in these cells by ectopic expression of GADD34 by retroviral transduction. These findings suggest that GADD34 contributes to the regulation of p21 expression, and that it suppresses cellular proliferation through the induction of cellular senescence.
Journal Cover

June 2007
Volume 17 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

2012 Impact Factor: 2.297
Ranked #36/196 Oncology
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APA
Minami, K., Inoue, H., Terashita, T., Kawakami, T., Watanabe, R., Haneda, M., Isobe, K., Okabe, H., & Chano, T. (2007). GADD34 induces p21 expression and cellular senescence. Oncology Reports, 17(6), 1481-1485.
MLA
Minami, Inoue, Terashita, Kawakami, Watanabe, Haneda, Isobe, Okabe, and Tokuhiro Chano. "GADD34 induces p21 expression and cellular senescence." Oncology Reports Oncology Reports 17.6 (2007): 1481-1485.
Chicago
Minami, Inoue, Terashita, Kawakami, Watanabe, Haneda, Isobe, Okabe, and Tokuhiro Chano. "GADD34 induces p21 expression and cellular senescence." Oncology Reports Oncology Reports 17 no. 6 (2007): 1481-1485.