In vivo and in vitro uptake of 111In, delivered with the affibody molecule (ZEGFR:955)2, in EGFR expressing tumour cells

  • Authors:
    • E. Nordberg
    • A. Orlova
    • M. Friedman
    • V. Tolmachev
    • S. Ståhl
    • F. Y. Nilsson
    • B. Glimelius
    • J. Carlsson
  • View Affiliations

  • Published online on: April 1, 2008     https://doi.org/10.3892/or.19.4.853
  • Pages: 853-857
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Abstract

The epidermal growth factor receptor, EGFR, is overexpressed in many carcinomas. Targeting this receptor with radionuclides is important for imaging and therapy applications in nuclear medicine. We investigated the in vitro and in vivo properties of a new high affinity EGFR binding affibody molecule, (ZEGFR:955)2, when conjugated with CHX-A''-DTPA and labelled with 111In. The binding time patterns and retention studies were performed using cultured squamous carcinoma A431 cells that overexpress EGFR. In the in vivo studies, female BALB/c nu/nu mice carrying tumours from xenografted A431 cells were used. The in vitro studies showed EGFR specific binding, high uptake and good retention of 111In when delivered as [111In](ZEGFR:955)2. The retention after 72 h of incubation was 38.0±1.15% of the initial level. The biodistribution study showed a tumour specific 111In uptake of 3.8±1.4% of injected dose per gram tumour tissue 4 h post-injection. The tumour to blood ratio was 9.1 and the tumours could easily be visualized with a gamma camera at this time-point. 111In delivered with [111In](ZEGFR:955)2 gave an EGFR specific uptake and the results indicated that the (ZEGFR:955)2 affibody molecule is a candidate for radionuclide-based tumour imaging. Potential therapy applications are discussed.

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April 2008
Volume 19 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Nordberg E, Orlova A, Friedman M, Tolmachev V, Ståhl S, Nilsson FY, Glimelius B and Carlsson J: In vivo and in vitro uptake of 111In, delivered with the affibody molecule (ZEGFR:955)2, in EGFR expressing tumour cells. Oncol Rep 19: 853-857, 2008.
APA
Nordberg, E., Orlova, A., Friedman, M., Tolmachev, V., Ståhl, S., Nilsson, F.Y. ... Carlsson, J. (2008). In vivo and in vitro uptake of 111In, delivered with the affibody molecule (ZEGFR:955)2, in EGFR expressing tumour cells. Oncology Reports, 19, 853-857. https://doi.org/10.3892/or.19.4.853
MLA
Nordberg, E., Orlova, A., Friedman, M., Tolmachev, V., Ståhl, S., Nilsson, F. Y., Glimelius, B., Carlsson, J."In vivo and in vitro uptake of 111In, delivered with the affibody molecule (ZEGFR:955)2, in EGFR expressing tumour cells". Oncology Reports 19.4 (2008): 853-857.
Chicago
Nordberg, E., Orlova, A., Friedman, M., Tolmachev, V., Ståhl, S., Nilsson, F. Y., Glimelius, B., Carlsson, J."In vivo and in vitro uptake of 111In, delivered with the affibody molecule (ZEGFR:955)2, in EGFR expressing tumour cells". Oncology Reports 19, no. 4 (2008): 853-857. https://doi.org/10.3892/or.19.4.853