Avemar, a nontoxic fermented wheat germ extract, attenuates the growth of sensitive and 5-FdUrd/Ara-C cross-resistant H9 human lymphoma cells through induction of apoptosis

  • Authors: Philipp Saiko, Maria Ozsvar-Kozma, Geraldine Graser, Andreas Lackner, Michael Grusch, Sibylle Madlener, Georg Krupitza, Walter Jaeger, Mate Hidvegi, Ram P. Agarwal, Monika Fritzer-Szekeres, Thomas Szekeres
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  • Published online on: Sunday, March 1, 2009
  • Pages: 787-791
  • DOI: 10.3892/or_00000285

Abstract

Avemar (MSC) is a nontoxic fermented wheat germ extract, which has been shown to significantly improve the survival rate in patients suffering from various malignancies. We investigated its effects in sensitive and 5-FdUrd/Ara-C cross-resistant H9 human lymphoma cells. After 48 and 72 h of incubation, Avemar inhibited the growth of sensitive H9 cells with IC50 values of 290 and 200 µg/ml, whereas the growth of 5-FdUrd/Ara-C cross-resistant H9 cells was attenuated with IC50 values of 180 and 145 µg/ml, respectively. Treatment with 300 µg/ml MSC for 48 h caused dose-dependent induction of apoptosis in 48% of sensitive H9 cells. In cross-resistant H9 cells, incubation with 200 µg/ml Avemar for 48 h led to 41% of apoptotic tumor cells. Growth arrest of sensitive H9 cells after exposure to various concentrations of MSC occurred mainly in the S phase of the cell cycle, thereby increasing the cell population from 54 to 73% while depleting cells in the G0-G1 phase from 40 to 19%. Growth arrest in cross-resistant H9 cells occurred also mainly in the S phase, increasing the cell population from 45 to 68% while depleting cells in the G0-G1 phase from 45 to 31%. As MSC treatment likely overcomes 5-FdUrd/Ara-C resistance, further investigations to elucidate the exact mechanisms are warranted. We conclude that Avemar exerts a number of beneficial effects which could support conventional chemotherapy of human malignancies.
Journal Cover

March 2009
Volume 21 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

2013 Impact Factor: 2.191
Ranked #33/202 Oncology
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APA
Saiko, P., Ozsvar-Kozma, M., Graser, G., Lackner, A., Grusch, M., Madlener, S., Krupitza, G., Jaeger, W., Hidvegi, M., Agarwal, R., Fritzer-Szekeres, M., & Szekeres, T. (2009). Avemar, a nontoxic fermented wheat germ extract, attenuates the growth of sensitive and 5-FdUrd/Ara-C cross-resistant H9 human lymphoma cells through induction of apoptosis. Oncology Reports, 21(3), 787-791.
MLA
Saiko, Ozsvar-Kozma, Graser, Lackner, Grusch, Madlener, Krupitza, Jaeger, Hidvegi, Agarwal, Fritzer-Szekeres, and Thomas Szekeres. "Avemar, a nontoxic fermented wheat germ extract, attenuates the growth of sensitive and 5-FdUrd/Ara-C cross-resistant H9 human lymphoma cells through induction of apoptosis." Oncology Reports Oncology Reports 21.3 (2009): 787-791.
Chicago
Saiko, Ozsvar-Kozma, Graser, Lackner, Grusch, Madlener, Krupitza, Jaeger, Hidvegi, Agarwal, Fritzer-Szekeres, and Thomas Szekeres. "Avemar, a nontoxic fermented wheat germ extract, attenuates the growth of sensitive and 5-FdUrd/Ara-C cross-resistant H9 human lymphoma cells through induction of apoptosis." Oncology Reports Oncology Reports 21 no. 3 (2009): 787-791.