Methylation of CLDN6, FBN2, RBP1, RBP4, TFPI2, and TMEFF2 in esophageal squamous cell carcinoma

  • Authors:
    • Shigeru Tsunoda
    • Eric Smith
    • Neville J. De Young
    • Xian Wang
    • Zi-Qing Tian
    • Jun-Feng Liu
    • Glyn G. Jamieson
    • Paul A. Drew
  • Corresponding author:
  • View Affiliations

  • Published online on: Wednesday, April 1, 2009
  • Pages: 1067-1073 DOI: 10.3892/or_00000325

Abstract

In the development and progression of cancer, tumor suppressor genes may be silenced by mechanisms such as methylation. Thus the discovery of new genes silenced by methylation may uncover new tumor suppressor genes, and improve our understanding of cancer biology. In this study we investigated the methylation of 19 genes in esophageal squamous cell carcinoma. Methylation was measured in 10 of these genes in esophageal squamous cell carcinoma cell lines: CDH13, CLDN6, C16orf62, FBN2, FNBP1, ID4, RBP1, RBP4, TFPI2 and TMEFF2. To determine if there was a correlation between DNA methylation and gene silencing, each cell line was cultured with or without the demethylating drug 5-aza-2'-deoxycytidine (aza-dC). For 6 genes (CLDN6, FBN2, RBP1, RBP4, TFPI2 and TMEFF2) there was an association between reduction of methylation and increase in mRNA expression in the demethylated cell lines. The frequency of the methylation of these 6 genes in esophageal squamous cell carcinoma resection specimens was also investigated. All 6 genes showed more frequent methylation in the tumor than the matched proximal resection margin of uninvolved esophagus. There was a significant difference in the frequency of methylation and in the extent of the methylation between the cancer and the margin tissues for CLDN6, FBN2, TFPI2 and TMEFF2 (P=0.0007, P=0.0048 P=0.0002 and P<0.0001, respectively). This is the first report of gene silencing by methylation of CLDN6, FBN2, RBP4, TFPI2 and TMEFF2 in esophageal squamous cell carcinoma.
Journal Cover

April 2009
Volume 21 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

2014 Impact Factor: 2.301
Ranked #31/211 Oncology
(total number of cites)

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APA
Tsunoda, S., Smith, E., De Young, N.J., Wang, X., Tian, Z., Liu, J. ... Drew, P.A. (2009). Methylation of CLDN6, FBN2, RBP1, RBP4, TFPI2, and TMEFF2 in esophageal squamous cell carcinoma. Oncology Reports, 21, 1067-1073. http://dx.doi.org/10.3892/or_00000325
MLA
Tsunoda, S., Smith, E., De Young, N. J., Wang, X., Tian, Z., Liu, J., Jamieson, G. G., Drew, P. A."Methylation of CLDN6, FBN2, RBP1, RBP4, TFPI2, and TMEFF2 in esophageal squamous cell carcinoma". Oncology Reports 21.4 (2009): 1067-1073.
Chicago
Tsunoda, S., Smith, E., De Young, N. J., Wang, X., Tian, Z., Liu, J., Jamieson, G. G., Drew, P. A."Methylation of CLDN6, FBN2, RBP1, RBP4, TFPI2, and TMEFF2 in esophageal squamous cell carcinoma". Oncology Reports 21, no. 4 (2009): 1067-1073. http://dx.doi.org/10.3892/or_00000325