Evidence for increased expression of tissue factor and protease-activated receptor-1 in human esophageal cancer

  • Authors: Fabiana S. Ribeiro, Tatiana A. Simão, Nivea D. Amoêdo, Nelson A. Andreollo, Luiz Roberto Lopes, Rodolfo Acatauassu, Franklin D. Rumjanek, Rodolpho M. Albano, Luis Felipe R. Pinto, Robson Q. Monteiro
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  • Published online on: Monday, June 1, 2009
  • Pages: 1599-1604
  • DOI: 10.3892/or_00000393

Abstract

It has been suggested that the blood clotting initiator protein, tissue factor (TF), participates in tumor growth, metastasis and angiogenesis. In addition, a family of G protein-coupled-receptors known as protease-activated receptors (PARs) has also been implicated in tumor biology. These receptors might be activated by blood coagulation proteases thus eliciting a number of pro-tumoral responses, including the expression of interleukin-8 (IL-8). Therefore, in this study we analyzed the expression of TF, PAR-1, PAR-2 and IL-8 genes in patients with esophageal cancer, one of the most aggressive neoplastic diseases. Total RNA was extracted from tissue samples (tumor and the corresponding normal mucosa) obtained from patients submitted to esophagectomy or endoscopy and further analyzed by semi-quantitative reverse transcriptase-polymerase (RT-PCR) and/or real-time quantitative PCR (qPCR). Expression of full-length transmembrane TF was significantly higher in tumor samples whereas no differences were observed in alternatively spliced TF transcripts. Tumor tissue showed increased mRNA levels for PAR-1 but not PAR-2. Remarkably, IL-8 expression was not detected in most normal tissues but showed very high expression in tumor samples. As expected, qPCR revealed greater differences in the expression pattern of all transcripts analyzed but the general profile was very similar to that observed by RT-PCR. Altogether our data suggest a possible role for blood clotting proteins in the biology of human esophageal cancer.
Journal Cover

June 2009
Volume 21 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

2013 Impact Factor: 2.191
Ranked #33/202 Oncology
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APA
Ribeiro, F., Simão, T., Amoêdo, N., Andreollo, N., Lopes, L., Acatauassu, R., Rumjanek, F., Albano, R., Pinto, L., & Monteiro, R. (2009). Evidence for increased expression of tissue factor and protease-activated receptor-1 in human esophageal cancer. Oncology Reports, 21(6), 1599-1604.
MLA
Ribeiro, Simão, Amoêdo, Andreollo, Lopes, Acatauassu, Rumjanek, Albano, Pinto, and Robson Monteiro. "Evidence for increased expression of tissue factor and protease-activated receptor-1 in human esophageal cancer." Oncology Reports Oncology Reports 21.6 (2009): 1599-1604.
Chicago
Ribeiro, Simão, Amoêdo, Andreollo, Lopes, Acatauassu, Rumjanek, Albano, Pinto, and Robson Monteiro. "Evidence for increased expression of tissue factor and protease-activated receptor-1 in human esophageal cancer." Oncology Reports Oncology Reports 21 no. 6 (2009): 1599-1604.