Cancerous, but not stromal, thrombospondin-2 contributes prognosis in pulmonary adenocarcinoma

  • Authors:
    • Tsuyoshi Chijiwa
    • Yoshiyuki Abe
    • Yoshimasa Inoue
    • Hozumi Matsumoto
    • Kenji Kawai
    • Masahiro Matsuyama
    • Noriyuki Miyazaki
    • Hiroshi Inoue
    • Masaya Mukai
    • Yoshito Ueyama
    • Masato Nakamura
  • Corresponding author:
  • View Affiliations

  • Published online on: Saturday, August 1, 2009
  • Pages: 279-283 DOI: 10.3892/or_00000435

Abstract

Thrombospondin (TSP)-2 is known to be an endogenous negative regulator of vascularization in human cancer. However, it is unclear whether TSP-2 expression is related to neovascularization and prognosis in non-small cell lung cancer. In this study, we quantitatively examined the expression of TSP-2 mRNA by real-time reverse transcription-polymerase chain reaction (RT-PCR) in 102 pulmonary adenocarcinomas. All 102 carcinoma specimens expressed TSP-2 mRNA. The expression of TSP-2 mRNA in carcinoma was significantly higher than normal lung tissues (p<0.0001, Kruskal-Wallis test). Sizes of tumors were significantly correlated with TSP-2 gene expression (p=0.0179, Kruskal-Wallis test). The TSP-2 expression levels of the stage II/III pulmonary carcinomas were significantly increased as compared to those of stage I (p=0.0136, Kruskal-Wallis test). Thirty-five patients with high TSP-2 mRNA expression showed poor prognosis in survival (p=0.0139, log-rank test). We examined TSP-2 protein localizations in the pulmonary adenocarcinoma overexpressing TSP-2 mRNA. The TSP-2 localizations were categorized in two patterns: cancerous TSP-2 expression pattern (TSP-2 expression in the cancerous cells) and non-cancerous TSP-2 expression pattern (TSP-2 expression in the stromal lymphoid cells). Pulmonary adenocarcinoma patients with cancerous TSP-2 expression pattern showed good prognosis (p=0.0322; Fisher's probability exact test). Pulmonary adenocarcinoma patients with non-cancerous TSP-2 expression pattern showed poor prognosis (p=0.0220; Fisher's probability exact test). Non-cancerous TSP-2 expressions may reflect secondary reactions in the cancerous stroma. The stromal TSP-2 expression is not enough to suppress growth of pulmonary adenocarcinoma, while the cancerous TSP-2 expression directly inhibits growth of the carcinoma.
Journal Cover

August 2009
Volume 22 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

2014 Impact Factor: 2.301
Ranked #31/211 Oncology
(total number of cites)

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APA
Chijiwa, T., Abe, Y., Inoue, Y., Matsumoto, H., Kawai, K., Matsuyama, M. ... Nakamura, M. (2009). Cancerous, but not stromal, thrombospondin-2 contributes prognosis in pulmonary adenocarcinoma. Oncology Reports, 22, 279-283. http://dx.doi.org/10.3892/or_00000435
MLA
Chijiwa, T., Abe, Y., Inoue, Y., Matsumoto, H., Kawai, K., Matsuyama, M., Miyazaki, N., Inoue, H., Mukai, M., Ueyama, Y., Nakamura, M."Cancerous, but not stromal, thrombospondin-2 contributes prognosis in pulmonary adenocarcinoma". Oncology Reports 22.2 (2009): 279-283.
Chicago
Chijiwa, T., Abe, Y., Inoue, Y., Matsumoto, H., Kawai, K., Matsuyama, M., Miyazaki, N., Inoue, H., Mukai, M., Ueyama, Y., Nakamura, M."Cancerous, but not stromal, thrombospondin-2 contributes prognosis in pulmonary adenocarcinoma". Oncology Reports 22, no. 2 (2009): 279-283. http://dx.doi.org/10.3892/or_00000435