Antitumor effects of a water-soluble extract from Maitake (Grifola frondosa) on human gastric cancer cell lines

  • Authors:
    • Kohei Shomori
    • Mako Yamamoto
    • Ichiro Arifuku
    • Kazuki Teramachi
    • Hisao Ito
  • View Affiliations

  • Published online on: Tuesday, September 1, 2009
  • Pages: 615-620
  • DOI: 10.3892/or_00000480

Abstract

We investigated the effects of a water-soluble extract of Maitake (Grifola frondosa), a Japanese edible mushroom, on the proliferation and cell death of four human gastric cancer cell lines (TMK-1, MKN28, MKN45 and MKN74). The Maitake extract (ME) inhibited the proliferation of all four cell lines in a time-dependent manner. The inhibition was most pronounced in TMK-1 cells, which exhibited up to 90% inhibition after treatment with 10% ME for 3 days. Staining of ME-treated TMK-1 cells with Hoechst 33258 revealed increased numbers of nuclear condensations and apoptotic bodies. Induction of apoptosis was confirmed by fluorescence-activated cell sorting analyses. Western blot analyses of TMK-1 cells after ME treatment revealed increases in intracytoplasmic cytochrome c and cleavage of caspase-3 and poly(ADP-ribose) polymerase, but no expression of p21 or Bax. The caspase-3 protease activities in lysates of TMK-1 cells treated with 1% or 10% ME were about three times higher than those in control cells. The proliferation of TMK-1 cells was hardly affected by the caspase-3 inhibitor z-DEVD-fmk. Taken together, these results suggest that ME induces apoptosis of TMK-1 cells by caspase-3-dependent and -independent pathways, resulting in potential antitumor effects on gastric cancer.
Journal Cover

September 2009
Volume 22 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

2013 Impact Factor: 2.191
Ranked #33/202 Oncology
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APA
Shomori, K., Yamamoto, M., Arifuku, I., Teramachi, K., & Ito, H. (2009). Antitumor effects of a water-soluble extract from Maitake (Grifola frondosa) on human gastric cancer cell lines. Oncology Reports, 22(3), 615-620.
MLA
Shomori, Yamamoto, Arifuku, Teramachi, and Hisao Ito. "Antitumor effects of a water-soluble extract from Maitake (Grifola frondosa) on human gastric cancer cell lines." Oncology Reports Oncology Reports 22.3 (2009): 615-620.
Chicago
Shomori, Yamamoto, Arifuku, Teramachi, and Hisao Ito. "Antitumor effects of a water-soluble extract from Maitake (Grifola frondosa) on human gastric cancer cell lines." Oncology Reports Oncology Reports 22 no. 3 (2009): 615-620.