Houttuynia cordata Thunb extract induces apoptosis through mitochondrial-dependent pathway in HT-29 human colon adenocarcinoma cells

  • Authors: Yih-Jing Tang, Jai-Sing Yang, Chin-Fen Lin, Woei-Cherng Shyu, Minoru Tsuzuki, Chi-Cheng Lu, Yuh-Fung Chen, Kuang-Chi Lai
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  • Published online on: Sunday, November 1, 2009
  • Pages: 1051-1056
  • DOI: 10.3892/or_00000535

Abstract

The Houttuynia cordata Thunb (HCT) extract has been used as a traditional Chinese herb medicine and as well as an effective drug for treating allergic inflammation for thousands of years. In this study, we investigated the anti-cancer activity of HCT and its molecular mechanisms in the human colon adenocarcinoma cell line HT-29. HCT inhibited HT-29 cell viability in a dose- and time-dependent manner by MTT assay. Treatment with 450 µg/ml of HCT for 48 and 72 h led to DNA damage and apoptosis by DAPI staining and comet assay. HCT increased reactive oxygen species production and decreased the levels of mitochondria membrane potential (MMP) in HT-29 cells by flow cytometry analysis. HCT caused the release of cytochrome c, Apaf-1, pro-caspase-9 and AIF from mitochondria via a decrease of the MMP. The decrease of MMP was then associated with a decrease in the ratio of Bax/Bcl-2 and activation of caspase-9 and -3 by Western blotting and caspase activity assay. Caspase-9 and -3 inhibitors almost completely suppressed HCT-induced caspase-9 and -3 activities. Our results demonstrated that the HCT-induced apoptosis in human colon adenocarcinoma cell line HT-29 might be related to a mitochondrial-dependent pathway.
Journal Cover

November 2009
Volume 22 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

2013 Impact Factor: 2.191
Ranked #33/202 Oncology
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APA
Tang, Y., Yang, J., Lin, C., Shyu, W., Tsuzuki, M., Lu, C., Chen, Y., & Lai, K. (2009). Houttuynia cordata Thunb extract induces apoptosis through mitochondrial-dependent pathway in HT-29 human colon adenocarcinoma cells. Oncology Reports, 22(5), 1051-1056.
MLA
Tang, Yang, Lin, Shyu, Tsuzuki, Lu, Chen, and Kuang-Chi Lai. "Houttuynia cordata Thunb extract induces apoptosis through mitochondrial-dependent pathway in HT-29 human colon adenocarcinoma cells." Oncology Reports Oncology Reports 22.5 (2009): 1051-1056.
Chicago
Tang, Yang, Lin, Shyu, Tsuzuki, Lu, Chen, and Kuang-Chi Lai. "Houttuynia cordata Thunb extract induces apoptosis through mitochondrial-dependent pathway in HT-29 human colon adenocarcinoma cells." Oncology Reports Oncology Reports 22 no. 5 (2009): 1051-1056.