Inhibitory effects of melatonin on sulfatase and 17β-hydroxysteroid dehydrogenase activity and expression in glioma cells

  • Authors:
    • Alicia González
    • Carlos Martínez-Campa
    • Maria Dolores Mediavilla
    • Carolina Alonso-González
    • Virginia Álvarez-García
    • Emilio José Sánchez-Barceló
    • Samuel Cos
  • View Affiliations

  • Published online on: April 1, 2010     https://doi.org/10.3892/or_00000748
  • Pages: 1173-1178
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Abstract

Melatonin interacts with estradiol at the estrogen receptor level in different kinds of neoplasias and also regulates the expression and the activity of some enzymes involved in the biosynthesis of estrogens in peripheral tissues. Glioma cells express estrogen receptors and have the ability to synthesize estrogens locally. Since melatonin inhibits the growth of C6 cells, and this indoleamine has been demonstrated to be capable of decreasing aromatase expression and activity in these cells, the aim of the present study was to analyze whether the regulation of the sulfatase, the enzyme that catalyzes the rate-limiting step in the conversion of estrogen sulfates to estrogens, and 17β-hydroxysteroid dehydrogenase, the enzyme which converts the relatively inactive estrone to the most potent 17β-estradiol, could be involved in the inhibition of glioma cell growth by melatonin. We found that melatonin decreases the growth of C6 glioma cells and reduces the sulfatase and 17β-hydroxysteroid dehydrogenase activity. Finally, we demonstrated that melatonin downregulates sulfatase and 17β-hydroxysteroid dehydrogenase mRNA steady state levels in these glioma cells. By analogy to the implications of these enzymes in other forms of estrogen-sensitive tumors, it is conceivable that their modulation by melatonin may play a role in the growth of glioblastomas.

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April 2010
Volume 23 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
González A, Martínez-Campa C, Mediavilla MD, Alonso-González C, Álvarez-García V, Sánchez-Barceló EJ and Cos S: Inhibitory effects of melatonin on sulfatase and 17β-hydroxysteroid dehydrogenase activity and expression in glioma cells. Oncol Rep 23: 1173-1178, 2010.
APA
González, A., Martínez-Campa, C., Mediavilla, M.D., Alonso-González, C., Álvarez-García, V., Sánchez-Barceló, E.J., & Cos, S. (2010). Inhibitory effects of melatonin on sulfatase and 17β-hydroxysteroid dehydrogenase activity and expression in glioma cells. Oncology Reports, 23, 1173-1178. https://doi.org/10.3892/or_00000748
MLA
González, A., Martínez-Campa, C., Mediavilla, M. D., Alonso-González, C., Álvarez-García, V., Sánchez-Barceló, E. J., Cos, S."Inhibitory effects of melatonin on sulfatase and 17β-hydroxysteroid dehydrogenase activity and expression in glioma cells". Oncology Reports 23.4 (2010): 1173-1178.
Chicago
González, A., Martínez-Campa, C., Mediavilla, M. D., Alonso-González, C., Álvarez-García, V., Sánchez-Barceló, E. J., Cos, S."Inhibitory effects of melatonin on sulfatase and 17β-hydroxysteroid dehydrogenase activity and expression in glioma cells". Oncology Reports 23, no. 4 (2010): 1173-1178. https://doi.org/10.3892/or_00000748