Activation of caspases and apoptosis in response to low-voltage electric pulses

  • Authors:
    • Noriaki Matsuki
    • Motohiro Takeda
    • Takuji Ishikawa
    • Aki Kinjo
    • Tomoaki Hayasaka
    • Yohsuke Imai
    • Takami Yamaguchi
  • View Affiliations

  • Published online on: May 1, 2010     https://doi.org/10.3892/or_00000780
  • Pages: 1425-1433
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Abstract

Few studies have examined apoptosis induced by low-voltage electric pulses (LVEPs). LVEP-induce changes in membrane potential that are below the membrane breakdown threshold and increase membrane permeability without electroporation (pore formation) through the transport of extracellular substances via phagocytosis. We demonstrated that propidium iodide uptake and apoptosis increased in accordance with the duration and number of LVEPs in B16 cells, which showed relatively good viability under mild electric field conditions. We showed that LVEP-induced apoptosis was achieved through caspase-8 and -9 activation and subsequent caspase-3 activation. Long-duration LVEPs caused only mild cell damage, such that the apoptosis ratio (apoptosis/total cell death) in electric pulse-treated cells was similar to that in non-treated control cells. To assess the relative degree of caspase dependency in LVEP-induced apoptosis, the apoptosis rate and caspase-3 activity were analyzed using a pan-caspase inhibitor (Z-VAD-FMK). Z-VAD-FMK treatment inhibited, but did not abolish, LVEP-induced apoptosis, indicating that caspases other than caspase-3 participate in this pathway. Moreover, LVEP treatment inhibited cell growth, suggesting that LVEP treatment may be a valuable anticancer therapy. Although the mechanism of LVEP-induced apoptosis remains unclear, it may be related to dysfunctional membrane transport of Ca2+ and other extracellular substances involved in caspase activation.

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May 2010
Volume 23 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Matsuki N, Takeda M, Ishikawa T, Kinjo A, Hayasaka T, Imai Y and Yamaguchi T: Activation of caspases and apoptosis in response to low-voltage electric pulses . Oncol Rep 23: 1425-1433, 2010.
APA
Matsuki, N., Takeda, M., Ishikawa, T., Kinjo, A., Hayasaka, T., Imai, Y., & Yamaguchi, T. (2010). Activation of caspases and apoptosis in response to low-voltage electric pulses . Oncology Reports, 23, 1425-1433. https://doi.org/10.3892/or_00000780
MLA
Matsuki, N., Takeda, M., Ishikawa, T., Kinjo, A., Hayasaka, T., Imai, Y., Yamaguchi, T."Activation of caspases and apoptosis in response to low-voltage electric pulses ". Oncology Reports 23.5 (2010): 1425-1433.
Chicago
Matsuki, N., Takeda, M., Ishikawa, T., Kinjo, A., Hayasaka, T., Imai, Y., Yamaguchi, T."Activation of caspases and apoptosis in response to low-voltage electric pulses ". Oncology Reports 23, no. 5 (2010): 1425-1433. https://doi.org/10.3892/or_00000780