Oridonin induces G2/M cell cycle arrest and apoptosis through MAPK and p53 signaling pathways in HepG2 cells

  • Authors:
    • Hui Wang
    • Yan Ye
    • Jian-Hong Chu
    • Guo-Yuan Zhu
    • Ying-Wei Li
    • David W.F. Fong
    • Zhi-Ling Yu
  • View Affiliations

  • Published online on: September 1, 2010     https://doi.org/10.3892/or_00000903
  • Pages: 647-651
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Abstract

Oridonin, the main active constituent of Isodon rubescen, has antihepatocarcinoma activity in experimental and clinical settings. The aims of the study were to explore the anticancer effect of oridonin in HepG2 cells and to investigate the underlying mechanisms. Results showed that oridonin treatment for 24 or 48 h resulted in a marked decrease in cell viability time- and dose-dependently. IC50 values were determined as 38.86 µM and 24.90 µM for 24-h and 48-h treatments, respectively. Flow cytometric analysis showed that a 24-h treatment of 40 µM oridonin induced G2/M cell cycle arrest and apoptosis. Typical apoptotic nucleus alterations were observed with fluorescence microscope after DAPI staining. Immunoblot analysis demonstrated that oridonin treatment increased expression levels of p-JNK, p-p38, p-p53 and p21, elevated the level of cyclin B1/p-Cdc2 (Tyr15) complex, and inhibited the expression of p-ERK. Moreover, oridonin treatment activated caspase-9 and caspase-3. In conclusion, oridonin induced G2/M cell cycle arrest and apoptosis in HepG2 cells through MAPK and p53 pathways, which advances our understanding on the molecular mechanisms of oridonin in hepatocarcinoma management.

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September 2010
Volume 24 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Wang H, Ye Y, Chu J, Zhu G, Li Y, Fong DW and Yu Z: Oridonin induces G2/M cell cycle arrest and apoptosis through MAPK and p53 signaling pathways in HepG2 cells . Oncol Rep 24: 647-651, 2010.
APA
Wang, H., Ye, Y., Chu, J., Zhu, G., Li, Y., Fong, D.W., & Yu, Z. (2010). Oridonin induces G2/M cell cycle arrest and apoptosis through MAPK and p53 signaling pathways in HepG2 cells . Oncology Reports, 24, 647-651. https://doi.org/10.3892/or_00000903
MLA
Wang, H., Ye, Y., Chu, J., Zhu, G., Li, Y., Fong, D. W., Yu, Z."Oridonin induces G2/M cell cycle arrest and apoptosis through MAPK and p53 signaling pathways in HepG2 cells ". Oncology Reports 24.3 (2010): 647-651.
Chicago
Wang, H., Ye, Y., Chu, J., Zhu, G., Li, Y., Fong, D. W., Yu, Z."Oridonin induces G2/M cell cycle arrest and apoptosis through MAPK and p53 signaling pathways in HepG2 cells ". Oncology Reports 24, no. 3 (2010): 647-651. https://doi.org/10.3892/or_00000903