14-3-3σ expression as a prognostic marker in undifferentiated nasopharyngeal carcinoma

  • Authors: Sylvia Yat-Yee Chan, Ka-Fai To, Sing-Fai Leung, William Wai-Lun Yip, Michael Ko-Fung Mak, Grace Tin-Yun Chung, Kwok-Wai Lo
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  • Published online on: Friday, October 1, 2010
  • Pages: 949-955
  • DOI: 10.3892/or_00000941

Abstract

In this study, we investigated the expression of 14-3-3σ tumor suppressor gene in a panel of NPC cell lines, xenografts and primary tumors. Our objective was to determine the correlation between 14-3-3σ expression and clinical outcome in NPC. We detected reduced 14-3-3σ expression in 5/6 NPC tumor lines by quantitative RT-PCR and Western blotting. By immunohistochemical staining, significant down-regulation of 14-3-3σ was also found in 26/72 (36.1%) primary tumors of NPC patients, who were treated with curative radiotherapy. Promoter methylation was confirmed in a subset of primary tumors by methylation-specific PCR analysis. Importantly, we demonstrated that 14-3-3σ expression is significantly associated with both overall survival (OS) and cancer-specific survival (CSS), but not with the clinical staging of NPC patients. The low 14-3-3σ expression was associated with improved overall (p=0.029) and cancer-specific survival (p=0.042) on univariate analysis. 14-3-3σ expression and staging were also independent variables to all the prognostic factors under multivariate analysis. In conclusion, low expression of 14-3-3σ appears to be a valuable marker for better survival in patient with NPC. These results provide the evidence that 14-3-3σ expression is a significant prognostic factor for NPC patients.
Journal Cover

October 2010
Volume 24 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

2013 Impact Factor: 2.191
Ranked #33/202 Oncology
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APA
Chan, S., To, K., Leung, S., Yip, W., Mak, M., Chung, G., & Lo, K. (2010). 14-3-3σ expression as a prognostic marker in undifferentiated nasopharyngeal carcinoma . Oncology Reports, 24(4), 949-955.
MLA
Chan, To, Leung, Yip, Mak, Chung, and Kwok-Wai Lo. "14-3-3σ expression as a prognostic marker in undifferentiated nasopharyngeal carcinoma ." Oncology Reports Oncology Reports 24.4 (2010): 949-955.
Chicago
Chan, To, Leung, Yip, Mak, Chung, and Kwok-Wai Lo. "14-3-3σ expression as a prognostic marker in undifferentiated nasopharyngeal carcinoma ." Oncology Reports Oncology Reports 24 no. 4 (2010): 949-955.