Adenovirus-mediated SOCS3 gene transfer inhibits the growth and enhances the radiosensitivity of human non-small cell lung cancer cells

  • Authors:
    • Yu-Ching Lin
    • Chin-Kuo Lin
    • Ying-Huang Tsai
    • Hsu-Huei Weng
    • Ya-Chin Li
    • Liang You
    • Jan-Kan Chen
    • David M. Jablons
    • Cheng-Ta Yang
  • View Affiliations

  • Published online on: Wednesday, December 1, 2010
  • Pages: 1605-1612
  • DOI: 10.3892/or_00001024

Abstract

The Janus kinase-signal transducers and activators of transcription (JAK-STAT) pathway is one of the most important components of cytokine signaling cascades. JAK-STAT signaling pathway modulates various fundamental biological processes and cancer pathogenesis. JAK-STAT is controlled by negative regulators that include suppressors of cytokine signaling (SOCS) proteins. Failure of feedback suppression by SOCS proteins may result in activated JAK-STAT signaling. Methylation-mediated silencing of SOCS3 has been reported in non-small lung cancer (NSCLC) and other human cancers. In this study, we restored SOCS3 expression using adenovirus-mediated gene transfer in NSCLC cells. Infection with a SOCS3-expressing vector inhibited the growth of lung cancer cells, with or without SOCS3 expression, at 2-3 days after infection. The growth inhibition of lung cancer cells was associated with suppressing entry into the S-phase. Restoration of SOCS3 expression induced apoptosis of NSCLC cells that did not express SOCS3. In addition, overexpression of SOCS3 by adenoviral transfer enhanced the radiosensitivity of treated NSCLC cells. In conclusion, our findings may provide insights into the development of applications of SOCS3 gene therapy for lung cancer and, possibly, other human cancers.
Journal Cover

December 2010
Volume 24 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

2013 Impact Factor: 2.191
Ranked #33/202 Oncology
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APA
Lin, Y., Lin, C., Tsai, Y., Weng, H., Li, Y., You, L., Chen, J., Jablons, D., & Yang, C. (2010). Adenovirus-mediated SOCS3 gene transfer inhibits the growth and enhances the radiosensitivity of human non-small cell lung cancer cells . Oncology Reports, 24(6), 1605-1612.
MLA
Lin, Lin, Tsai, Weng, Li, You, Chen, Jablons, and Cheng-Ta Yang. "Adenovirus-mediated SOCS3 gene transfer inhibits the growth and enhances the radiosensitivity of human non-small cell lung cancer cells ." Oncology Reports Oncology Reports 24.6 (2010): 1605-1612.
Chicago
Lin, Lin, Tsai, Weng, Li, You, Chen, Jablons, and Cheng-Ta Yang. "Adenovirus-mediated SOCS3 gene transfer inhibits the growth and enhances the radiosensitivity of human non-small cell lung cancer cells ." Oncology Reports Oncology Reports 24 no. 6 (2010): 1605-1612.