Tumor-infiltrating CD4+ Th17 cells produce IL-17 in tumor microenvironment and promote tumor progression in human gastric cancer

  • Authors: Takeshi Iida, Makoto Iwahashi, Masahiro Katsuda, Koichiro Ishida, Mikihito Nakamori, Masaki Nakamura, Teiji Naka, Toshiyasu Ojima, Kentaro Ueda, Keiji Hayata, Yasushi Nakamura, Hiroki Yamaue
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  • Published online on: Tuesday, March 1, 2011
  • Pages: 1271-1277
  • DOI: 10.3892/or.2011.1201

Abstract

Recently, a subset of IL-17 producing T cells distinct from Th1 or Th2 cells has been described as key players in inflammation and autoimmune diseases as well as cancer development. In this study, we investigated the expression level of IL-17 and T helper 17 (Th17)-related cytokines in gastric cancer tissues and assessed the association of their expression with angiogenesis and their clinicopathological parameters. Tumor and adjacent normal tissues were obtained from 82 patients with gastric cancer. IL-17, IL-21 and IL-23 mRNA expression levels were quantified by real-time RT-PCR. Th17 infiltration, microvessel density and neutrophil infiltration in tumor tissues were examined by immunohistochemistry and double immunofluorescence histochemistry. Expression of IL-17, IL-21 and IL-23 mRNA was found to be significantly up-regulated in tumor tissues compared with adjacent normal tissues. The expression level of IL-17 mRNA strongly and positively correlated with that of IL-21 mRNA in tumor tissue. The number of vascular endothelial cells and infiltrating neutrophils was significantly larger in tumors expressing a high level of IL-17 mRNA than in tumors expressing a low level of IL-17 mRNA. In tumor tissues most CD4+ cells were stained with anti-IL-17 antibody. The expression level of IL-17 mRNA in gastric tumors was associated with the depth of the tumors, lymph-vascular invasion and lymph node involvement, suggesting that IL-17 obviously was related to tumor progression. IL-17 and IL-21, which regulates IL-17, would be potential therapeutic targets for the treatment of gastric cancer.
Journal Cover

May 2011
Volume 25 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

2012 Impact Factor: 2.297
Ranked #36/196 Oncology
(total number of cites)

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APA
Iida, T., Iwahashi, M., Katsuda, M., Ishida, K., Nakamori, M., Nakamura, M., Naka, T., Ojima, T., Ueda, K., Hayata, K., Nakamura, Y., & Yamaue, . (2011). Tumor-infiltrating CD4+ Th17 cells produce IL-17 in tumor microenvironment and promote tumor progression in human gastric cancer. Oncology Reports, 25(5), 1271-1277.
MLA
Iida, Iwahashi, Katsuda, Ishida, Nakamori, Nakamura, Naka, Ojima, Ueda, Hayata, Nakamura, and Hiroki Yamaue. "Tumor-infiltrating CD4+ Th17 cells produce IL-17 in tumor microenvironment and promote tumor progression in human gastric cancer." Oncology Reports Oncology Reports 25.5 (2011): 1271-1277.
Chicago
Iida, Iwahashi, Katsuda, Ishida, Nakamori, Nakamura, Naka, Ojima, Ueda, Hayata, Nakamura, and Hiroki Yamaue. "Tumor-infiltrating CD4+ Th17 cells produce IL-17 in tumor microenvironment and promote tumor progression in human gastric cancer." Oncology Reports Oncology Reports 25 no. 5 (2011): 1271-1277.