Expression of KL-6/MUC1 in pancreatic cancer tissues and its potential involvement in tumor metastasis

  • Authors:
    • Huanli Xu
    • Yoshinori Inagaki
    • Yasuji Seyama
    • Guanhua Du
    • Fengshan Wang
    • Norihiro Kokudo
    • Wei Tang
  • View Affiliations

  • Published online on: Monday, May 23, 2011
  • Pages: 371-376
  • DOI: 10.3892/or.2011.1315

Abstract

Aberrant expression of KL-6/MUC1 mucin has been proven to be associated with poorer tumor behavior in many carcinomas. The aim of this study was to evaluate the expression of KL-6/MUC1 in pancreatic cancer tissues and its potential involvement in tumor metastasis. The expression of KL-6/MUC1 in 18 cases of pancreatic ductal carcinoma (PDC), 5 cases of intraductal papillary mucinous tumor (IPMT), and 3 cases of islet cell tumor was detected by immunohistochemical staining. To determine the impact of loss of KL-6/MUC1 expression on pancreatic tumor progression, an siRNA targeting MUC1 was synthesized and transfected into Panc-1 cells and Capan-1 cells to knock down KL-6/MUC1 expression. Down-regulation of KL-6/MUC1 expression was detected by reverse transcription-polymerase chain reaction (RT-PCR) and western blotting. E-cadherin and KL-6 mucin co-expression was detected by immunofluorescence. The expression of E-cadherin and E-cadherin/β-catenin comlex was determined by immunoprecipitation. Cell invasive abilities were detected by invasion assay. Positive KL-6/MUC1 staining was observed in all 18 PDC cases (18/18,100.0%) and 1 metastatic IPMT case (1/5, 20.0%). The results suggested that KL-6/MUC1 overexpression may be associated with more aggressive tumor behavior, although the cases of pancreatic cancer tissues in this study are limited. RT-PCR and western blotting showed that both KL-6/MUC1 mRNA and protein can be effectively silenced. Following KL-6/MUC1 knockdown, E-cadherin expression increased. Also, E-cadherin/β-catenin complex expression increased and the invasive ability of the cells decreased. This study indicated that overexpression of KL-6/MUC1 in pancreatic cancer tissues may be associated with metastasis of pancreatic cancer by regulating E-cadherin and E-cadherin/β-catenin complex expression.
Journal Cover

August 2011
Volume 26 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

2013 Impact Factor: 2.191
Ranked #33/202 Oncology
(total number of cites)

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APA
Xu, H., Inagaki, Y., Seyama, Y., Du, G., Wang, F., Kokudo, N., & Tang, W. (2011). Expression of KL-6/MUC1 in pancreatic cancer tissues and its potential involvement in tumor metastasis. Oncology Reports, 26(2), 371-376.
MLA
Xu, Inagaki, Seyama, Du, Wang, Kokudo, and Wei Tang. "Expression of KL-6/MUC1 in pancreatic cancer tissues and its potential involvement in tumor metastasis." Oncology Reports Oncology Reports 26.2 (2011): 371-376.
Chicago
Xu, Inagaki, Seyama, Du, Wang, Kokudo, and Wei Tang. "Expression of KL-6/MUC1 in pancreatic cancer tissues and its potential involvement in tumor metastasis." Oncology Reports Oncology Reports 26 no. 2 (2011): 371-376.