β-catenin plays a key role in metastasis of human hepatocellular carcinoma

  • Authors:
    • Tung-Yuan Lai
    • Cheng-Chuan Su
    • Wei-Wen Kuo
    • Yu-Lan Yeh
    • Wu-Hsien Kuo
    • Fuu-Jen Tsai
    • Chang-Hai Tsai
    • Yi-Jiun Weng
    • Chih-Yang Huang
    • Li-Mien Chen
  • View Affiliations

  • Published online on: May 26, 2011     https://doi.org/10.3892/or.2011.1323
  • Pages: 415-422
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Abstract

Currently, there are no diagnostic or metastatic markers that can be used in early diagnosis and treatment of human hepatocellular carcinoma (HCC). The aim of this study was to find a molecular marker that regulated migration and metastasis in HCC. We analyzed the gene expression of β-catenin, c-Myc and IL-8 in human HCC tissue by RT-PCR and immunohistochemistry and analyzed five variously differentiated HCC cell lines by Western blotting and migration and invasion assays to find markers for HCC diagnosis and HCC metastasis. mRNA expression of β-catenin was significantly higher in the tumor area compared to the non-tumor area and was more abundant in specimens of late-stage HCC. Immunohistochemistry revealed that the translocation of β-catenin into the nucleus was closely correlated with IL-8 protein levels and tumor stage. Similarly, the level of expression and nuclear translocation of β-catenin was greater in HA22T cells with high proliferative activity than in HCC cell lines with low proliferative activity (PLC, Hep3B, HepG2). Knockdown of the β-catenin gene with β-catenin antisense oligonucleotides resulted in inhibition of cell migration and invasion of HA22T cells. Taken together, these results suggest that β-catenin may be a suitable diagnostic marker of metastasis in human HCC.

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August 2011
Volume 26 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Lai T, Su C, Kuo W, Yeh Y, Kuo W, Tsai F, Tsai C, Weng Y, Huang C, Chen L, Chen L, et al: β-catenin plays a key role in metastasis of human hepatocellular carcinoma. Oncol Rep 26: 415-422, 2011
APA
Lai, T., Su, C., Kuo, W., Yeh, Y., Kuo, W., Tsai, F. ... Chen, L. (2011). β-catenin plays a key role in metastasis of human hepatocellular carcinoma. Oncology Reports, 26, 415-422. https://doi.org/10.3892/or.2011.1323
MLA
Lai, T., Su, C., Kuo, W., Yeh, Y., Kuo, W., Tsai, F., Tsai, C., Weng, Y., Huang, C., Chen, L."β-catenin plays a key role in metastasis of human hepatocellular carcinoma". Oncology Reports 26.2 (2011): 415-422.
Chicago
Lai, T., Su, C., Kuo, W., Yeh, Y., Kuo, W., Tsai, F., Tsai, C., Weng, Y., Huang, C., Chen, L."β-catenin plays a key role in metastasis of human hepatocellular carcinoma". Oncology Reports 26, no. 2 (2011): 415-422. https://doi.org/10.3892/or.2011.1323