Nestin expression in high-grade osteosarcomas and its clinical significance

  • Authors:
    • Iva Zambo
    • Marketa Hermanova
    • Dagmar Adamkova Krakorova
    • Peter Mudry
    • Karel Zitterbart
    • Michal Kyr
    • Karel Vesely
    • Jaroslav Sterba
    • Renata Veselska
  • View Affiliations

  • Published online on: Tuesday, February 14, 2012
  • Pages: 1592-1598
  • DOI: 10.3892/or.2012.1687

Abstract

Nestin has been detected in various malignancies and its expression correlates with advanced grade in some neoplasms. The aim of this study was to examine nestin expression in high-grade osteosarcomas and to determine its prognostic value. Using immunohistochemistry and immunofluorescence, we evaluated nestin expression in tumor tissue samples from 45 patients with high-grade osteosarcomas. In both methods, the frequency of nestin-positive tumor cells was classified into three categories (1+, 2+ and 3+ for immunohistochemistry; 1F+, 2F+ and 3F+ for immunofluorescence) and clinicopathological correlations were statistically evaluated and analyzed. Nestin-positive tumor cells were detected in all of the examined osteosarcomas using both immunohistochemistry and immunofluorescence, although the proportion of undoubtedly positive neoplastic cells varied in individual samples from a few nestin-positive tumor cells to diffuse nestin positivity. High levels of nestin expression detected by immunofluorescence (2F+ and 3F+) were associated with worse clinical outcomes (OS, p=0.031; EFS, p<0.001). However, high levels of nestin expression as measured by immunohistochemistry trended towards shorter patient survival rates but did not reach statistical significance. Despite significantly shorter survival rates observed in patients with high levels of nestin expression assessed by immunofluorescence, nestin does not seem to represent a powerful prognostic marker that would be superior to conventional methods.
Journal Cover

May 2012
Volume 27 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

2013 Impact Factor: 2.191
Ranked #33/202 Oncology
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Zambo, I., Hermanova, M., Adamkova Krakorova, D., Mudry, P., Zitterbart, K., Kyr, M., Vesely, K., Sterba, J., & Veselska, R. (2012). Nestin expression in high-grade osteosarcomas and its clinical significance. Oncology Reports, 27(5), 1592-1598.
MLA
Zambo, Hermanova, Adamkova Krakorova, Mudry, Zitterbart, Kyr, Vesely, Sterba, and Renata Veselska. "Nestin expression in high-grade osteosarcomas and its clinical significance." Oncology Reports Oncology Reports 27.5 (2012): 1592-1598.
Chicago
Zambo, Hermanova, Adamkova Krakorova, Mudry, Zitterbart, Kyr, Vesely, Sterba, and Renata Veselska. "Nestin expression in high-grade osteosarcomas and its clinical significance." Oncology Reports Oncology Reports 27 no. 5 (2012): 1592-1598.