Induction of fibronectin in response to epidermal growth factor is suppressed by silibinin through the inhibition of STAT3 in triple negative breast cancer cells

  • Authors:
    • Sangmin Kim
    • Myeongjin Jeon
    • Jeongmin Lee
    • Jeonghun Han
    • Soo‑Jin  Oh
    • Taewoo Jung
    • Seok Jin Nam
    • Won Ho Kil
    • Jeong Eon Lee
  • View Affiliations

  • Published online on: August 29, 2014     https://doi.org/10.3892/or.2014.3450
  • Pages: 2230-2236
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Abstract

Fibronectin (FN) plays a major role in cell adhesion, migration and oncogenic transformation. Aberrant FN expression is associated with poor prognosis in various types of cancer, including breast cancer. In this study, we investigated the effect of silibinin on the epidermal growth factor (EGF)-induced FN expression in triple negative breast cancer (TNBC) cells. Our data showed that the levels of FN mRNA and protein expression were dose-dependently increased by EGF in MDA-MB468 and BT20 breast cancer cells. Consequently, EGF-induced FN expression was decreased by the epidermal growth factor receptor (EGFR) inhibitors AG1478 and gefitinib. EGF-induced FN expression was also decreased by MEK1/2, PI3K and STAT3 specific inhibitors. In the present study, we observed for the first time that EGF-induced FN expression was significantly decreased by silibinin treatment in TNBC cells. Furthermore, we found that silibinin suppressed the EGF-induced phosphorylation of STAT3 but not Erk and Akt. Taken together, silibinin downregulated EGF-induced FN expression through the inhibition of STAT3 phosphorylation in TNBC cells. Silibinin may be a promising anticancer drug for the treatment of TNBC.
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November-2014
Volume 32 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Kim S, Jeon M, Lee J, Han J, Oh SJ, Jung T, Nam SJ, Kil WH and Lee JE: Induction of fibronectin in response to epidermal growth factor is suppressed by silibinin through the inhibition of STAT3 in triple negative breast cancer cells. Oncol Rep 32: 2230-2236, 2014
APA
Kim, S., Jeon, M., Lee, J., Han, J., Oh, S., Jung, T. ... Lee, J.E. (2014). Induction of fibronectin in response to epidermal growth factor is suppressed by silibinin through the inhibition of STAT3 in triple negative breast cancer cells. Oncology Reports, 32, 2230-2236. https://doi.org/10.3892/or.2014.3450
MLA
Kim, S., Jeon, M., Lee, J., Han, J., Oh, S., Jung, T., Nam, S. J., Kil, W. H., Lee, J. E."Induction of fibronectin in response to epidermal growth factor is suppressed by silibinin through the inhibition of STAT3 in triple negative breast cancer cells". Oncology Reports 32.5 (2014): 2230-2236.
Chicago
Kim, S., Jeon, M., Lee, J., Han, J., Oh, S., Jung, T., Nam, S. J., Kil, W. H., Lee, J. E."Induction of fibronectin in response to epidermal growth factor is suppressed by silibinin through the inhibition of STAT3 in triple negative breast cancer cells". Oncology Reports 32, no. 5 (2014): 2230-2236. https://doi.org/10.3892/or.2014.3450