Relationship between expression of Livin and the biological behavior of human oral squamous cell carcinoma

Lee,Dong Hoon; Yoon,Tae Mi; Kim,Sun-Ae; Park,Young-Lan; Lee,Kyung-Hwa; Lim,Sang Chul; Lee,Joon Kyoo; Joο,Young Eun

doi:10.3892/or.2014.3510

Relationship between expression of Livin and the biological behavior of human oral squamous cell carcinoma

Authors:
- Dong Hoon Lee
- Tae Mi Yoon
- Sun-Ae Kim
- Young-Lan Park
- Kyung-Hwa Lee
- Sang Chul Lim
- Joon Kyoo Lee
- Young Eun Joο
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Affiliations: Department of Otorhinolaryngology - Head and Neck Surgery, Chonnam National University Medical School, Research Institute of Medical Sciences, Chonnam National University and Hwasun Hospital, Hwasun 519-809, Republic of Korea, Department of Internal Medicine, Chonnam National University Medical School, Research Institute of Medical Sciences, Chonnam National University and Hwasun Hospital, Hwasun 519-809, Republic of Korea, Department of Pathology, Chonnam National University Medical School, Research Institute of Medical Sciences, Chonnam National University and Hwasun Hospital, Hwasun 519-809, Republic of Korea
Published online on: September 22, 2014 https://doi.org/10.3892/or.2014.3510
Pages: 2453-2460

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Abstract

Livin is one of the most important members of the inhibitor protein (IAP) family. It is overexpressed in several types of tumors and may have prognostic significance. The purpose of this study was to investigate Livin expression in human oral squamous cell carcinomas (OSCCs) and to determine whether Livin affects tumor cell behavior in OSCC cell lines and thus evaluate its potential usefulness in serving as a possible target for molecular-targeted therapy in a preclinical model. The expression of Livin protein was investigated in human OSCC tissues through immunohistochemistry and western blot analysis. To evaluate the impact of Livin knockdown on the behavior of human OSCC cell lines, invasion, migration, proliferation and apoptosis assays using small-interfering RNA (siRNA) were performed. RT-PCR and western blot analysis were used to assess alteration of Livin expression at the mRNA and protein levels. The results revealed that expression of Livin was increased in the human OSCC tissues compared with the adjacent normal mucosa. In addition, immunoreactivity of Livin was expressed in 8 OSCC tissues (44.4%). Knockdown of Livin resulted in significantly reduced cell invasion, migration and proliferation in the human OSCC cells. Livin knockdown induced cell apoptosis in the human OSCC cells. Moreover, Livin inhibited apoptosis by suppressing the activity of caspases in the human OSCC cells. In conclusion, livin is associated with invasive and oncogenic phenotypes such as tumor cell invasion, tumor cell migration, tumor cell proliferation, and resistance to apoptosis in human OSCC cells.

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