Elevated expression level of long noncoding RNA MALAT-1 facilitates cell growth, migration and invasion in pancreatic cancer

Jiao,Feng; Hu,Hai; Yuan,Cuncun; Wang,Lei; Jiang,Weihua; Jin,Ziliang; Guo,Zhen; Wang,Liwei

doi:10.3892/or.2014.3518

Elevated expression level of long noncoding RNA MALAT-1 facilitates cell growth, migration and invasion in pancreatic cancer

Authors:
- Feng Jiao
- Hai Hu
- Cuncun Yuan
- Lei Wang
- Weihua Jiang
- Ziliang Jin
- Zhen Guo
- Liwei Wang
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Affiliations: Department of Oncology and Shanghai Key Laboratory of Pancreatic Diseases, First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 201620, P.R. China, Department of Pathology, First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 201620, P.R. China
Published online on: September 26, 2014 https://doi.org/10.3892/or.2014.3518
Pages: 2485-2492

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Abstract

Pancreatic cancer is one of the most aggressive solid malignancies with a dismal survival rate. Recent studies have shown that high expression levels of long noncoding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript-1 (MALAT-1) correlate with several solid tumors. However, the underlying molecular mechanisms and its clinical significance in pancreatic cancer remain to be elucidated. In the present study, our results showed that MALAT-1 expression levels were upregulated in pancreatic cancer tissues compared with adjacent noncancerous controls. Consistently, higher expression level of MALAT-1 was found in all seven pancreatic cancer cell lines relative to the human pancreatic ductal epithelial cell. Further function analysis revealed that downregulation of MALAT-1 could inhibit tumor cell proliferation and decrease cell migration and invasion in vitro. The underlying mechanisms are possibly involved in inducing G2/M cell cycle arrest, promoting cell apoptosis, suppressing epithelial-mesenchymal transition and reducing cancer stem-like properties. In conclusion, this study indicated that MALAT-1 may serve as an oncogenic lncRNA that is involved in malignancy phenotypes of pancreatic cancer. Therefore, it may be used as a potential therapeutic target.

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