Restoration of miR-7 expression suppresses the growth of Lewis lung cancer cells by modulating epidermal growth factor receptor signaling

Li,Jingrong; Zheng,Yijie; Sun,Gengyun; Xiong,Shudao

doi:10.3892/or.2014.3519

Restoration of miR-7 expression suppresses the growth of Lewis lung cancer cells by modulating epidermal growth factor receptor signaling

Authors:
- Jingrong Li
- Yijie Zheng
- Gengyun Sun
- Shudao Xiong
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Affiliations: Department of Emergency, The Second Hospital of Anhui Medical University, Hefei, Anhui, P.R. China, Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, NY, USA, Department of Respiratory Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei, P.R. China, Department of Hematology/Oncology, The Second Hospital of Anhui Medical University, Hefei, Anhui, P.R. China
Published online on: September 26, 2014 https://doi.org/10.3892/or.2014.3519
Pages: 2511-2516

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Abstract

microRNAs are an abundant class of short endogenous non-coding RNAs that function as important regulators of multiple target genes and participate in diverse biological roles in carcinogenesis. However, the role of miR-7 in lung cancer remains unclear and requires further elucidation. In the present study, we found a reduction of miR-7 expression in Lewis lung cancer (3LL) cells originating from mice by real-time RT-PCR. Restoration of miR-7 inhibited 3LL cell proliferation, induced cell apoptosis in vitro and reduced tumorigenicity in vivo. We further confirmed that miR-7 downregulated the expression of both epidermal growth factor receptor (EGFR) and murine leukemia viral oncogene homologue-1 (RAF-1) oncogenes by real-time PCR and western blot analysis. Furthermore, inhibition of EGFR showed similar effects to miR-7 enforcement in 3LL cells. Taken together, these findings revealed that miR-7 acts as an antitumor miRNA in 3LL by targeting and suppressing the expression of both EGFR and RAF-1 oncogenes. This study may provide a rationale for the use of miR-7 in lung cancer target therapy.

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