Ethyl acetate extract from Jiedu Xiaozheng Yin inhibits the proliferation of human hepatocellular carcinoma cells by suppressing polycomb gene product Bmi1 and Wnt/β-catenin signaling

  • Authors:
    • Xu-Zheng Chen
    • Zhi-Yun Cao
    • Jin-Nong Li
    • Hai-Xia Hu
    • You-Quan Zhang
    • Yun-Mei Huang
    • Zhi-Zhen Liu
    • Dan Hu
    • Lian-Ming Liao
    • Jian Du
  • View Affiliations

  • Published online on: October 13, 2014     https://doi.org/10.3892/or.2014.3541
  • Pages: 2710-2718
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Abstract

Jiedu Xiaozheng Yin (JXY) is a Chinese herbal decoction used to treat hepatocellular carcinoma (HCC). Previous studies have demonstrated that JXY can inhibit HCC cell proliferation via induction of G0/G1 phase arrest. In this study, we investigated whether the inhibitory effect of JXY on HCC cells is associated with the inhibition of the Wnt/β‑catenin pathway and the polycomb gene product Bmi1. Ethyl acetate extract from JXY (EE-JXY) was prepared. Methyl thiazolyl tetrazolium (MTT) and colony formation assays were used to measure cell proliferation. Immunofluorescence was used to analyze the expression and location of β-catenin and Bmi1. Immunohistochemistry was used to examine the expression of proliferating cell nuclear antigen (PCNA), c-myc and cyclin D1. β-catenin, Bmi1, c-myc, cyclin D1 and p16INK4A mRNA levels were detected by RT-PCR. The results demonstrated that EE-JXY inhibited the expression of PCNA, c-myc, cyclin D1 and Bmi1, and upregulated the expression of p16INK4A. We also found that EE-JXY could facilitate β-catenin translocation from the cytoplasm and nuclei to the cytomembrane. Finally, suppression of cell proliferation and expression of Bmi1 and Wnt/β-catenin by EE-JXY was confirmed in a mouse xenograft model of HCC. Thus, EE-JXY can inhibit the proliferation of HCC partially via suppression of the Bmi1 and Wnt/β-catenin signaling pathways.
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December-2014
Volume 32 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Chen X, Cao Z, Li J, Hu H, Zhang Y, Huang Y, Liu Z, Hu D, Liao L, Du J, Du J, et al: Ethyl acetate extract from Jiedu Xiaozheng Yin inhibits the proliferation of human hepatocellular carcinoma cells by suppressing polycomb gene product Bmi1 and Wnt/β-catenin signaling. Oncol Rep 32: 2710-2718, 2014
APA
Chen, X., Cao, Z., Li, J., Hu, H., Zhang, Y., Huang, Y. ... Du, J. (2014). Ethyl acetate extract from Jiedu Xiaozheng Yin inhibits the proliferation of human hepatocellular carcinoma cells by suppressing polycomb gene product Bmi1 and Wnt/β-catenin signaling. Oncology Reports, 32, 2710-2718. https://doi.org/10.3892/or.2014.3541
MLA
Chen, X., Cao, Z., Li, J., Hu, H., Zhang, Y., Huang, Y., Liu, Z., Hu, D., Liao, L., Du, J."Ethyl acetate extract from Jiedu Xiaozheng Yin inhibits the proliferation of human hepatocellular carcinoma cells by suppressing polycomb gene product Bmi1 and Wnt/β-catenin signaling". Oncology Reports 32.6 (2014): 2710-2718.
Chicago
Chen, X., Cao, Z., Li, J., Hu, H., Zhang, Y., Huang, Y., Liu, Z., Hu, D., Liao, L., Du, J."Ethyl acetate extract from Jiedu Xiaozheng Yin inhibits the proliferation of human hepatocellular carcinoma cells by suppressing polycomb gene product Bmi1 and Wnt/β-catenin signaling". Oncology Reports 32, no. 6 (2014): 2710-2718. https://doi.org/10.3892/or.2014.3541