MicroRNA-182 inhibits proliferation through targeting oncogenic ANUBL1 in gastric cancer

Tang,Lin; Chen,Feng; Pang,Er-Jun; Zhang,Zhi-Qi; Jin,Bing-Wei; Dong,Wei-Feng

doi:10.3892/or.2015.3798

MicroRNA-182 inhibits proliferation through targeting oncogenic ANUBL1 in gastric cancer

Authors:
- Lin Tang
- Feng Chen
- Er-Jun Pang
- Zhi-Qi Zhang
- Bing-Wei Jin
- Wei-Feng Dong
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Affiliations: School of Basic Medical Sciences, Nanjing University of Chinese Medicine, Nanjing 210023, P.R. China, Division of General Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, P.R. China
Published online on: February 12, 2015 https://doi.org/10.3892/or.2015.3798
Pages: 1707-1716

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Abstract

MicroRNA-182 (miR-182) is significantly downregulated in human gastric tissue samples. Overexpression of miR-182 suppresses the proliferation and colony formation of gastric cancer cells. However, new aspects of the mechanism are still emerging in gastric cancer. ANUBL1, also known as ZFAND4 (zinc finger, AN1-type domain 4), its roles are scarely reported in cancer. In this study, we not only showed that ANUBL1 as an oncogene was upregulated and could promote proliferation of SGC-7901 cells, but also demonstrated that its overexpression led to a strong decrease of miR-182 expression and expression of ANUBL1 was in turn directly downregulated by miR-182, thereby establishing a negative feedback loop between miR-182 and ANUBL1. The elucidation of the mechanisms of miR-182 targeting ANUBL1 in gastric cancer helps us to further understand the mechanism of gastric cancer initiation and progression.

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